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PMID: 5821734 Published · ppublish English Journal Article

Kinetic complexity of chloroplastal deoxyribonucleic acid and mitochondrial deoxyribonucleic acid from higher plants.

The Biochemical journal ·Vol. 112 ·No. 5 ·1969-05-00 ·Pages 777-86

Wells R, Birnstiel M

Abstract

1. Chloroplasts and mitochondria were isolated by aqueous and non-aqueous cell-fractionation techniques. In a variety of higher plants the mitochondrial DNA bands in a caesium chloride gradient at 1.706g.cm.(-3), whereas chloroplastal DNA has a buoyant density of 1.697g.cm.(-3). 2. In total cellular DNA of moderate molecular weight, the chloroplastal DNA is found within the Gaussian distribution of the nuclear DNA and is not resolved as a satellite. 3. Both chloroplastal DNA and mitochondrial DNA from lettuce renature rapidly. 4. The kinetic complexity of mitochondrial DNA is > 10(8) daltons. 5. Chloroplastal DNA is made up from fast and slow renaturing sequences with kinetic complexities of 3x10(6) and 1.2x10(8) daltons respectively. 6. From the discrepancy between analytical and kinetic complexity it is concluded that chloroplastal DNA is extensively reiterated.

MeSH Terms
Cell Nucleus Centrifugation, Density Gradient Chloroplasts DNA/analysis DNA, Bacterial DNA, Viral Densitometry Kinetics Methods Microscopy, Electron Mitochondria Molecular Weight Nucleic Acid Denaturation Plant Cells Plants Purines/analysis Pyrimidinones/analysis Species Specificity Time Factors
Chemicals
DNA, Bacterial DNA, Viral Purines Pyrimidinones DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wells R
Birnstiel M
References (23)
23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1120777
Published
1969-05-00
Pages
777-86
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187784
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1120777 PMC Full Text View journal details
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