Investigation into rabbit infusion media for the growth of Mycoplasma gallisepticum antigens for inoculation into rabbits.
Serum cholesterol in rabbits was elevated, by intravenous inoculation of 20% Tween 80, to amounts thought to have been sufficient to support growth of Mycoplasma gallisepticum when 20% of such serum was added to rabbit infusion broth.However, better growth of the organism was obtained with a supplement of 5% rabbit serum and commercial cholesterol. Investigation showed that normal rabbit serum may be inhibitory, which would explain these findings. The commercial cholesterol was not antigenic.
20 references, click to expand
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A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production.J Hyg (Lond). 1966 Mar;64(1):91-104 PMID: 5219024
-
Effect of culture medium on antigenic structure of mycoplasma.Avian Dis. 1966 May;10(2):173-6 PMID: 5963855
-
Factors influencing growth inhibition of Mycoplasma pneumoniae by immune sera.Proc Soc Exp Biol Med. 1967 Oct;126(1):161-6 PMID: 6066157
-
Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum.J Comp Pathol. 1968 Oct;78(4):407-14 PMID: 5695834
-
[Use of the metabolic inhibition test (MI-test) for Mycoplasma gallisepticum].Dtsch Tierarztl Wochenschr. 1970 Jan 1;77(1):12-4 PMID: 5534809
-
Sustained hyperlipemia induced in rabbits by means of intravenously injected surface-active agents.J Exp Med. 1951 Apr 1;93(4):373-84 PMID: 14824409
-
The isolation of organisms of the pleuropneumonia group from dogs.J Gen Microbiol. 1951 Aug;5(3):566-75 PMID: 14873903
-
Cholesterol in the growth of organisms of the pleuropneumonia group.J Gen Microbiol. 1951 Aug;5(3):576-86 PMID: 14873904
-
The dilution assay of viruses.J Hyg (Lond). 1954 Jun;52(2):189-93 PMID: 13174799
-
A lipoprotein as a growth factor for cortain pleuropneumonialike organisms.J Bacteriol. 1954 Nov;68(5):627-33 PMID: 13211570
-
Another test for heterogeneity of host resistance in dilution assays.J Hyg (Lond). 1958 Sep;56(3):319-22 PMID: 13587982
-
The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of Mycoplasma mycoides.J Gen Microbiol. 1961 Jun;25:201-14 PMID: 13742641
-
CHOLESTEROL AND CHOLESTEROL ESTERS IN MYCOPLASMA.J Gen Microbiol. 1965 Jan;38:153-60 PMID: 14283030
-
EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS.J Immunol. 1965 Jul;95:19-25 PMID: 14322576
-
The classification and nomenclature of organisms of the pleuropneumonia group.J Gen Microbiol. 1956 Feb;14(1):197-207 PMID: 13306904
-
THE STEROID GROWTH-REQUIREMENT OF MYCOPLASMA MYCOIDES.J Gen Microbiol. 1963 Jul;32:91-101 PMID: 14045527
-
SEROLOGICAL RELATIONSHIPS AMONG HUMAN MYCOPLASMAS AS SHOWN BY COMPLEMENT-FIXATION AND GEL DIFFUSION.J Bacteriol. 1963 Jun;85:1261-73 PMID: 14047216
-
A new color reaction for the quantitation of serum cholesterol.Clin Chim Acta. 1960 Mar;5:192-9 PMID: 14444356
-
Nutritional requirements of PPLO and their relation to metabolic function.Ann N Y Acad Sci. 1960 Jan 15;79:508-20 PMID: 13832119
-
The survival of washed suspensions of Mycoplasma.J Gen Microbiol. 1960 Apr;22:470-7 PMID: 13806498
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