RNA polymerases of maize: nuclear RNA polymerases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 11 ·1971-11-00 ·Pages 2647-51

Strain GC, Mullinix KP, Bogorad L

Abstract

Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays. The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH(4))(2)SO(4), respectively. Both enzymes prefer maize nuclear DNA as a template; they are also more active in the presence of Mg(++) than Mn(++) and are inhibited by (NH(4))(2)-SO(4) or KCl. Neither enzyme is inhibited by rifamycin SV. Enzyme II is strongly inhibited by alpha-amanitin, whereas enzyme I is not significantly affected. Their ability to use native and denatured DNA as templates varies according to the extent and method of purification of the polymerase. Furthermore, enzyme II can be resolved by DEAE-chromatography or glycerol-gradient centrifugation into two components, one of which prefers native DNA, while the other prefers denatured DNA.

MeSH Terms

Ammonium Sulfate Centrifugation, Density Gradient Chromatography, DEAE-Cellulose DNA/isolation & purification Magnesium Manganese Potassium Chloride RNA Nucleotidyltransferases/antagonists & inhibitors,isolation & purification Rifampin Templates, Genetic Zea mays/enzymology

Chemicals

Manganese Potassium Chloride DNA RNA Nucleotidyltransferases Magnesium Ammonium Sulfate Rifampin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Strain G C

Mullinix K P

Bogorad L

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.11.2647

Published

1971-11-00

Pages

2647-51

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389492

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.11.2647 PMC Full Text View journal details

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RNA polymerases of maize: nuclear RNA polymerases.

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