Specific inhibitors of the three RNA polymerases from the aquatic fungus Blastocladiella emersonii.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 2 ·1971-02-00 ·Pages 338-41

Horgen PA, Griffin DH

Abstract

Specific inhibitors of each of the three RNA polymerases of Blastocladiella emersonii have been found. Cycloheximide specifically inhibited the in vitro activity of the DEAE-fraction I enzyme, alpha-amanitin specifically inhibited the DEAE-fraction II enzyme, and rifampicin specifically inhibited the fraction III enzyme. DNA stimulation and dependency on the four riboside triphosphates were shown to be characteristic of each of the three fractions. Optimum concentrations of magnesium ions required were shown to differ among the three fractions and to be somewhat higher than optimum concentrations of manganese ions. The effect of pH on activity was essentially identical for each of the three fractions. Kinetic experiments and nuclease assays indicated the presence of some interfering substances in the partially purified RNA polymerase fractions.

MeSH Terms

Biological Assay Blastomyces/enzymology Choline/pharmacology Cycloheximide/pharmacology

Chemicals

Cycloheximide Choline

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Horgen P A

Griffin D H

References (18)

18 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.2.338

Published

1971-02-00

Pages

338-41

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388932

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.2.338 PMC Full Text View journal details

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Specific inhibitors of the three RNA polymerases from the aquatic fungus Blastocladiella emersonii.

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