The rolling circle for phi X DNA replication. 3. Synthesis of supercoiled duplex rings.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 1 ·1970-09-00 ·Pages 456-63

Dressler D, Wolfson J

Abstract

During varphiX duplex ring synthesis, the first supercoils to acquire radioactivity after the addition of tritiated thymidine are labeled only in their negative strands. In longer pulses, this asymmetry of labeling progressively disappears. This finding supports the rolling circle model for DNA replication due to the structural asymmetry of its replicating intermediate, but is not predicted by the Cairns or Yoshikawa models.

MeSH Terms

Carbon Isotopes Centrifugation, Density Gradient Coliphages/metabolism DNA Replication Phosphorus Isotopes Thymidine Tritium

Chemicals

Carbon Isotopes Phosphorus Isotopes Tritium Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dressler D

Wolfson J

References (14)

14 references, click to expand

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Proc Natl Acad Sci U S A. 1969 Sep;64(1):275-82 PMID: 5263011
The twisted circular form of polyoma viral DNA.
Proc Natl Acad Sci U S A. 1965 May;53(5):1104-11 PMID: 4287964
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Proc Natl Acad Sci U S A. 1963 Aug;50:236-43 PMID: 14060639
Function of gene 32-protein, a new protein essential for the genetic recombination and replication of T4 bacteriophage DNA.
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Structure of the replicative form of bacteriphage phi X174. VI. Studies on alkali-denatured double-stranded phi X DNA.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.1.456

Published

1970-09-00

Pages

456-63

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283226

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.1.456 PMC Full Text View journal details

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The rolling circle for phi X DNA replication. 3. Synthesis of supercoiled duplex rings.

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