The process of infection with bacteriophage phiX174. XXX. Replication of double-stranded phiX DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 1 ·1969-09-00 ·Pages 275-82

Knippers R, Whalley JM, Sinsheimer RL

Abstract

Intermediates involved in the replication of double-stranded varphiX174 RF DNA have been identified and partially characterized. Analysis of pulselabeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with "tails" and "double rings," which could be the intermediate structures. A model is postulated for the replication process.

MeSH Terms

Binding Sites Coliphages DNA Replication DNA, Viral/analysis Microscopy, Electron Models, Chemical Templates, Genetic

Chemicals

DNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Knippers R

Whalley J M

Sinsheimer R L

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.1.275

Published

1969-09-00

Pages

275-82

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC286158

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.1.275 PMC Full Text View journal details

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The process of infection with bacteriophage phiX174. XXX. Replication of double-stranded phiX DNA.

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