Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis.

The Biochemical journal ·Vol. 121 ·No. 5 ·1971-03-00 ·Pages 817-27

Hider RC, Fern EB, London DR

Abstract

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

MeSH Terms

Amino Acids/analysis,metabolism Animals Carbon Isotopes Extracellular Space/metabolism Glycine/metabolism Half-Life In Vitro Techniques Inulin/metabolism Kinetics Leucine/metabolism Mannitol/metabolism Muscle Proteins/biosynthesis Muscles/metabolism Rats Sorbitol/metabolism Sulfates/metabolism Sulfur Isotopes Tritium

Chemicals

Amino Acids Carbon Isotopes Muscle Proteins Sulfates Sulfur Isotopes Tritium Mannitol Sorbitol Inulin Leucine Glycine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hider R C

Fern E B

London D R

References (23)

23 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1210817

Published

1971-03-00

Pages

817-27

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1176670

Subset

External Links

PubMed source DOI: 10.1042/bj1210817 PMC Full Text View journal details

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Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis.

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