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PMID: 5484664 Published · ppublish English Journal Article

The influence of electrical stimulation in vitro on protein synthesis and other metabolic parameters of rat extensor digitorum longus muscle.

The Biochemical journal ·Vol. 118 ·No. 2 ·1970-06-00 ·Pages 209-20

Pain VM, Manchester KL

Abstract

1. Apparatus is described in which rat extensor digitorum longus muscle can be incubated in buffer under conditions of light tension and be subject to contractures induced by electrical stimulation in vitro. Under these conditions the tissue retains its weight, its content of potassium and size of the extracellular space at values similar to those in vivo. 2. Though uptake of glucose was enhanced on addition of insulin, there was little increase in glucose consumption on stimulation. Breakdown of glycogen and enhancement of lactate output were found on stimulation. 3. Incorporation into protein of several labelled amino acids was diminished during stimulation. Accumulation of [(14)C]leucine was enhanced whereas that of glycine was decreased. 4. There were no very consistent changes in the content of free unlabelled amino acids during incubation with or without stimulation. Comparison of actual amino acid concentrations in tissue and incubation mixture with accumulation of (14)C-labelled amino acid indicated that full equilibration of the cell pool of amino amino acids with the medium is slow. 5. Substantial oxidation of several (14)C-labelled acids was observed. 6. The ATP content of the tissue declined a little during incubation and somewhat faster after a period of stimulation. 7. The results are discussed in relation to the way in which exercise can induce muscle hypertrophy.

MeSH Terms
Adenine Nucleotides/metabolism Adenosine Triphosphate/metabolism Amino Acids/metabolism Animals Electric Stimulation Female Glucose/metabolism Glycine/metabolism Glycogen/metabolism Hypertrophy/etiology,metabolism In Vitro Techniques Insulin/pharmacology Lactates/metabolism Leucine/metabolism Muscle Proteins/biosynthesis Muscles/drug effects,metabolism Rats
Chemicals
Adenine Nucleotides Amino Acids Insulin Lactates Muscle Proteins Adenosine Triphosphate Glycogen Leucine Glucose Glycine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pain V M
Manchester K L
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45 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1180209
Published
1970-06-00
Pages
209-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179105
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1180209 PMC Full Text View journal details
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