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PMID: 5113494 Published · ppublish English Journal Article

The interaction of concanavalin A with blood-group-substance glycoproteins from human secretions.

The Biochemical journal ·Vol. 121 ·No. 5 ·1971-03-00 ·Pages 811-6

Clarke AE, Denborough MA

Abstract

1. Gastric juice, saliva and ovarian-cyst fluid were fractionated into glycoprotein components by centrifuging to equilibrium in a caesium chloride density gradient. 2. The glycoprotein fractions from the gastric juice of two group O non-secretors, two group O secretors and three group A secretors all formed insoluble complexes with concanavalin A. 3. Fractions showing maximum interaction with concanavalin A had maximum blood-group activity measured by the haemagglutination-inhibition technique. The sulphate content of the gastric glycoproteins was unrelated to the capacity to interact with concanavalin A. 4. No interaction was found between concanavalin A and the glycoprotein fractions from any of the saliva or ovarian-cyst-fluid samples tested, implying that there is a structural difference in blood-group-substance glycoproteins in gastric juice when compared with those in saliva and ovarian-cyst fluid. 5. The protein components of each of the secretions tested, gastric juice, saliva and ovarian-cyst fluid, interacted with concanavalin A.

MeSH Terms
ABO Blood-Group System/metabolism Body Fluids/analysis Carbohydrates/analysis Centrifugation, Density Gradient Chemical Precipitation Concanavalin A Densitometry Female Gastric Juice/analysis Glycoproteins Hemagglutination Inhibition Tests Humans Lectins Lewis Blood Group Antigens Ovarian Cysts/immunology Saliva/analysis Sulfates/analysis
Chemicals
ABO Blood-Group System Carbohydrates Glycoproteins Lectins Lewis Blood Group Antigens Sulfates Concanavalin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Clarke A E
Denborough M A
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18 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1210811
Published
1971-03-00
Pages
811-6
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176669
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1210811 PMC Full Text View journal details
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