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PMID: 5098867 Published · ppublish English Journal Article

RNA metabolism in HeLa cells at reduced temperature. I. Modified processing of 45S RNA.

The Journal of cell biology ·Vol. 50 ·No. 3 ·1971-09-00 ·Pages 818-29

Stevens RH, Amos H

Abstract

Incubation of HeLa cells at suboptimal temperature has been used to study the synthesis of 45S ribosomal RNA precursor and the individual steps of the subsequent processing to 28S RNA. Below 20 degrees C no detectable 45S RNA is formed. The processing of 45S RNA to 32S RNA ceases around 15 degrees C, and the processing of 32S RNA to 28S RNA is inhibited near 25 degrees C. Prolonged incubation at reduced temperature results in further modification of the processing, resulting in the apparent accumulation of 41S RNA. The products of these reactions at reduced temperature appear normal in that the ribosomal RNA made at 27 degrees C can be isolated from functional polyribosomes in the cytoplasm after a short incubation at 37 degrees C.

MeSH Terms
Carbon Isotopes Cell Fractionation Cells, Cultured Electrophoresis HeLa Cells/metabolism Methionine/metabolism Methylation RNA, Neoplasm/analysis,metabolism RNA, Ribosomal/analysis,biosynthesis Ribosomes/analysis Temperature Time Factors Tritium Uracil Nucleotides/analysis,isolation & purification Uridine/metabolism
Chemicals
Carbon Isotopes RNA, Neoplasm RNA, Ribosomal Uracil Nucleotides Tritium Methionine Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stevens R H
Amos H
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22 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.50.3.818
Published
1971-09-00
Pages
818-29
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108309
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.50.3.818 PMC Full Text View journal details
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