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PMID: 5022177 Published · ppublish English Journal Article

Development of chicken lymphoid system. I. Synthesis and secretion of immunoglobulins by chicken lymphoid cells.

The Journal of experimental medicine ·Vol. 135 ·No. 5 ·1972-05-01 ·Pages 1133-50

Choi YS, Good RA

Abstract

Synthesis and secretion of Ig by chicken lymphoid cells was studied. Both spleen and bursa cells synthesize and secrete IgM and IgG whereas Ig was not detected in thymus cells. In contrast to the spleen cells which synthesize H and L chains in balanced quantities, the bursa cells synthesize and secrete free L chains. In addition to the lymphoid cells which secrete IgM or IgG, the bursa appears to contain a cell population which synthesizes nonsecretory Ig. The structure of this Ig was studied by specific serological precipitation and by SDS-acrylamide gel electrophoresis. The H chains of this nonsecretory Ig are serologically related to micro-chains and exhibit a smaller molecular weight (i.e., approximately 50,000) in SDS-acrylamide gel electrophoresis than H chains of IgG and IgM synthesized by the spleen cells (i.e., approximately 70,000).

MeSH Terms
Animals Bursa of Fabricius/immunology,metabolism Cells, Cultured Chickens Electrophoresis, Disc Immunoglobulin G/analysis,biosynthesis,metabolism Immunoglobulin M/analysis,biosynthesis,metabolism Immunoglobulins/biosynthesis,metabolism Leucine/metabolism Lymphoid Tissue/immunology,metabolism Methods Rabbits Spleen/immunology,metabolism Thymus Gland/immunology,metabolism Tritium
Chemicals
Immunoglobulin G Immunoglobulin M Immunoglobulins Tritium Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Choi Y S
Good R A
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31 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.135.5.1133
Published
1972-05-01
Pages
1133-50
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138975
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.135.5.1133 PMC Full Text View journal details
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