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PMID: 5003172 Published · ppublish English Journal Article

Detection of a rapidly metabolizing portion of the membrane cardiolipin in Haemophilus parainfluenzae.

Journal of bacteriology ·Vol. 108 ·No. 3 ·1971-12-00 ·Pages 1058-64

Tucker AN, White DC

Abstract

Heterogeneity in the metabolism of cardiolipin (CL) has been detected in Haemophilus parainfluenzae. Pulse-chase experiments showed that a portion of the total CL incorporated and then lost (32)P much more rapidly than the rest of the CL in the cells. The metabolism of each phosphate of the CL differed. The phosphate of the phosphatidyl glycerol (PG) portion of the CL had a more active metabolism than the phosphate of the phosphatidic acid portion of the molecule. Only a portion of the PG pool contributed to the formation of CL. Ethylenediaminetetraacetic acid inhibited the CL-specific phospholipase D in vitro and, when added to growing cells, resulted in more rapid PG metabolism, suggesting that CL hydrolysis contributed to the PG pool.

MeSH Terms
Autoradiography Cell Wall/analysis,metabolism Chromatography, Paper Edetic Acid/pharmacology Electron Transport Haemophilus/analysis,enzymology,growth & development,metabolism Hydrolysis Lipids/analysis Phosphates/metabolism Phosphatidylethanolamines/metabolism Phospholipases/antagonists & inhibitors,metabolism Phospholipids/analysis,metabolism Phosphorus Isotopes Spectrophotometry
Chemicals
Lipids Phosphates Phosphatidylethanolamines Phospholipids Phosphorus Isotopes Edetic Acid Phospholipases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tucker A N
White D C
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.108.3.1058-1064.1971
Published
1971-12-00
Pages
1058-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247188
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.108.3.1058-1064.1971 PMC Full Text View journal details
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