Identity of genes coding for soluble and structural dihydrofolate reductases in bacteriophage T4.

Journal of virology ·Vol. 7 ·No. 4 ·1971-04-00 ·Pages 531-3

Mathews CK

Abstract

By recombination between bacteriophage T4 wh2, a dihydrofolate reductaseless mutant, and T6, I have prepared T4 wh(T6), a T4 strain which codes for the T6-specific soluble dihydrofolate reductase. This strain has the heat sensitivity of T6, not T4, which provides direct evidence that the wh gene codes for both the soluble dihydrofolate reductase and the structural dihydrofolate reductase which is a constituent of T-even phage tail plates.

MeSH Terms

Adsorption Coliphages/enzymology,growth & development Crosses, Genetic Enzyme Repression Escherichia coli Genes Genetic Code Genetics, Microbial Hot Temperature Lysogeny Mutation Recombination, Genetic Tetrahydrofolate Dehydrogenase/metabolism Urea/pharmacology Viral Proteins

Chemicals

Viral Proteins Urea Tetrahydrofolate Dehydrogenase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Mathews C K

References (7)

7 references, click to expand

Linkage of T4 genes controlling a series of steps in pyrimidine biosynthesis.
Virology. 1967 Mar;31(3):442-8 PMID: 5336881
Mutants of bacteriophage T4 unable to induce dihydrofolate reductase activity.
Proc Natl Acad Sci U S A. 1967 Aug;58(2):584-91 PMID: 4860754
Evidence that bacteriophage-induced dihydrofolate reductase in a viral gene product.
J Biol Chem. 1967 Sep 25;242(18):4083-6 PMID: 4863042
Growth of a dihydrofolate reductaseless mutant of bacteriophage T4.
J Virol. 1967 Oct;1(5):963-7 PMID: 4912241
Bacteriophage tail components. I. Pteroyl polyglutamates in T-even bacteriophages.
J Virol. 1970 Jun;5(6):726-39 PMID: 4914098
Bacteriophage tail components. II. Dihydrofolate reductase in T4D bacteriophage.
J Virol. 1970 Jun;5(6):740-53 PMID: 4393396
COMPARATIVE BIOCHEMISTRY OF BACTERIAL AND PHAGE-INDUCED DIHYDROFOLATE REDUCTASES.
J Biol Chem. 1965 May;240:2142-7 PMID: 14299639

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.7.4.531-533.1971

Published

1971-04-00

Pages

531-3

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356154

Subset

External Links

PubMed source DOI: 10.1128/JVI.7.4.531-533.1971 PMC Full Text View journal details

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Identity of genes coding for soluble and structural dihydrofolate reductases in bacteriophage T4.

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