Biosynthesis of 4-aminobenzoate in Escherichia coli.

Journal of bacteriology ·Vol. 102 ·No. 3 ·1970-06-00 ·Pages 767-73

Huang M, Gibson F

Abstract

Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations. Two components with approximate molecular weights of 9,000 (component A) and 48,000 (component B) are concerned in the biosynthesis of 4-aminobenzoate from chorismate by E. coli. No diffusible intermediate compound could be detected.

MeSH Terms

Aminobenzoates/biosynthesis Cell-Free System Chromatography Chromosome Mapping Culture Media Cyclohexanecarboxylic Acids/metabolism Escherichia coli/enzymology,growth & development,metabolism Filtration Fluorometry Genetics, Microbial Guanidines Molecular Weight Mutagens Mutation Nitroso Compounds Penicillins Phosphotransferases/metabolism Transduction, Genetic Ultraviolet Rays

Chemicals

Aminobenzoates Culture Media Cyclohexanecarboxylic Acids Guanidines Mutagens Nitroso Compounds Penicillins Phosphotransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Huang M

Gibson F

References (21)

21 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.102.3.767-773.1970

Published

1970-06-00

Pages

767-73

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC247625

Subset

External Links

PubMed source DOI: 10.1128/jb.102.3.767-773.1970 PMC Full Text View journal details

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Biosynthesis of 4-aminobenzoate in Escherichia coli.

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