Transketolase mutants of Escherichia coli.

Journal of bacteriology ·Vol. 100 ·No. 3 ·1969-12-00 ·Pages 1289-95

Josephson BL, Fraenkel DG

Abstract

Transketolase mutants have been selected after ethyl methane sulfonate mutagenesis of Escherichia coli. These strains are unable to grow on any pentose and, in addition, require a supplement of aromatic amino acids or shikimic acid for normal growth on any other carbon source. Revertants are normal in both respects and also contain transketolase. Transketolase mutants do not require exogenous pentose for growth. Preliminary genetic mapping of the locus is presented.

MeSH Terms

Chromosome Mapping Conjugation, Genetic Escherichia coli/enzymology Glucose-6-Phosphate Isomerase/metabolism Glucosephosphate Dehydrogenase/metabolism Isomerases/metabolism Mutation Pentoses/metabolism Phosphogluconate Dehydrogenase/metabolism Ribose/metabolism Transferases/metabolism

Chemicals

Pentoses Ribose Phosphogluconate Dehydrogenase Glucosephosphate Dehydrogenase Transferases Isomerases Glucose-6-Phosphate Isomerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Josephson B L

Fraenkel D G

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.100.3.1289-1295.1969

Published

1969-12-00

Pages

1289-95

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC250317

Subset

External Links

PubMed source DOI: 10.1128/jb.100.3.1289-1295.1969 PMC Full Text View journal details

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