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PMID: 4745422 Published · ppublish English Journal Article

Chemical analysis of the outer membrane and other layers of the cell envelope of Acinetobacter sp.

Journal of bacteriology ·Vol. 116 ·No. 1 ·1973-10-00 ·Pages 410-7

Thorne KJ, Thornley MJ, Glauert AM

Abstract

Chemical analysis of fractions of the cell envelope of Acinetobacter sp. strain MJT/F5/199A, prepared by breakage in the French press and removal of plasma membranes, followed by sequential treatment with lysozyme and with papain, confirmed the existence of layers previously identified by electron microscopy. Outside the plasma membrane and periplasmic space, the envelope is composed of (i) a peptidoglycan-containing dense layer, (ii) an intermediate layer, (iii) a lipopolysaccharide-containing outer membrane, and (iv) an ordered array of protein subunits. A small amount of carbohydrate (3%) is found associated with protein in the fraction containing both the surface subunits and the intermediate layer. The papain-treated outer membranes contain 67% protein, 24% lipid, together with 11% lipopolysaccharide, and about 6% of non-lipopolysaccharide hexosamine. Lipid is located only in the papain-treated outer-membrane and is mainly phospholipid: 29% phosphatidyl glycerol, 30% phosphatidyl ethanolamine, and 40% cardiolipin. The principal fatty acid is C(18:1). Significant amounts of alcohols(16:1) and alcohols(18:1), which are found in Acinetobacter waxes, were recovered from the outer membrane.

MeSH Terms
Alcaligenes/analysis Bacterial Proteins/analysis Carbohydrates/analysis Cell Fractionation Cell Membrane/analysis Cell Wall/analysis Chromatography, Thin Layer Fatty Acids/analysis Hexosamines/analysis Hexoses/analysis Lipids/analysis Lipopolysaccharides/analysis Microscopy, Electron Muramidase Papain Peptidoglycan/analysis Phospholipids/analysis Pimelic Acids/analysis Polysaccharides, Bacterial/analysis Tritium
Chemicals
Bacterial Proteins Carbohydrates Fatty Acids Hexosamines Hexoses Lipids Lipopolysaccharides Peptidoglycan Phospholipids Pimelic Acids Polysaccharides, Bacterial Tritium Muramidase Papain
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thorne K J
Thornley M J
Glauert A M
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.116.1.410-417.1973
Published
1973-10-00
Pages
410-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC246437
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.116.1.410-417.1973 PMC Full Text View journal details
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