Serum-mediated leukemia cell destruction in AKR mice.

The Journal of experimental medicine ·Vol. 138 ·No. 4 ·1973-10-01 ·Pages 925-38

Kassel RL, Old LJ, Carswell EA, Fiore NC, Hardy WD

Abstract

AKR mice with spontaneous leukemia were infused with normal serum from a variety of species. Leukemia cell destruction was produced by serum from strains of mice possessing the full spectrum of complement components, but not by serum from strains with a genetically determined deficiency of C5. Serum from guinea pigs, horses, and humans also causes destruction of leukemia cells. The antileukemic factor in normal serum was heat labile (56 degrees C for 35 min) and could be inactivated by cobra venom factor (CVF). Tests of individual complement factors from guinea pig serum and from human serum suggest that C5 is the antileukemic complement component in normal serum. Evidence was obtained that complement also plays a role in the antileukemic effect of interferon and endotoxin.

MeSH Terms

Animals Antigen-Antibody Complex Complement System Proteins Disease Models, Animal Endotoxins Guinea Pigs Horses Humans Immune Sera Interferons Leukemia, Experimental/immunology,pathology Lymph Nodes/immunology Mice Mice, Inbred AKR Species Specificity Spleen/immunology Venoms

Chemicals

Antigen-Antibody Complex Endotoxins Immune Sera Venoms Complement System Proteins Interferons

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Kassel R L

Old L J

Carswell E A

Fiore N C

Hardy W D

References (25)

25 references, click to expand

Isolation of the anticomplementary protein from cobra venom and its mode of action on C3.
J Immunol. 1971 Dec;107(6):1666-72 PMID: 5120401
Serum complement level in patients with leukemia.
Int J Cancer. 1969 Nov 15;4(6):845-51 PMID: 5265376
Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum.
Immunochemistry. 1966 Mar;3(2):111-35 PMID: 5960883
Antigens of leukemias induced by naturally occurring murine leukemia virus: their relation to the antigens of gross virus and other murine leukemia viruses.
J Exp Med. 1966 Oct 1;124(4):753-72 PMID: 4288480
The G (Gross) leukemia antigen.
Cancer Res. 1965 Jul;25(6):813-9 PMID: 4284252
DISTRIBUTION, INHERITANCE, AND PROPERTIES OF AN ANTIGEN, MUB1, AND ITS RELATION TO HEMOLYTIC COMPLEMENT.
J Exp Med. 1964 Nov 1;120:897-924 PMID: 14247728
Carcinolytic effects of interferon.
Clin Obstet Gynecol. 1970 Dec;13(4):910-27 PMID: 5516579
Prevention of the localized and generalized Shwartzman reactions by an anticomplementary agent, cobra venom factor.
J Exp Med. 1971 Sep 1;134(3 Pt 1):642-55 PMID: 15776567
A study of passive immunization against a transplanted G+ leukemia with specific antiserum.
Proc Soc Exp Biol Med. 1967 Jan;124(1):63-8 PMID: 6017793
Wild-type Gross leukemia virus and the pathogenesis of the glomerulonephritis of New Zealand mice.
J Exp Med. 1971 Jan 1;133(1):113-32 PMID: 4924198
A SINGLE GENE CONTROLLING HEMOLYTIC COMPLEMENT AND A SERUM ANTIGEN IN THE MOUSE.
J Immunol. 1964 Apr;92:611-5 PMID: 14139033
Regression of transplanted lymphomas induced in vivo by means of normal guinea pig serum. II. Studies on the nature of the active serum constituent: histological mechanism of the regression: tests for effects of guinea pig serum on lymphoma cells in vitro: discussion.
J Exp Med. 1953 Dec;98(6):583-606 PMID: 13109111
Wild-type Gross leukemia virus. I. Soluble antigen (GSA) in the plasma and tissues of infected mice.
J Natl Cancer Inst. 1968 Jul;41(1):89-96 PMID: 4880891
Interferon.
Trans N Y Acad Sci. 1970 May;32(5):545-56 PMID: 5291224
Occurrence of natural antibody to the G (gross) leukemia antigen in mice.
Cancer Res. 1966 Jul;26(7):1415-9 PMID: 5911583
Interferon-mediated oncolysis in spontaneous murine leukemia.
J Natl Cancer Inst. 1972 Apr;48(4):1155-9 PMID: 5023676
Improved complementation in the cytotoxic test.
Transplantation. 1970 Nov;10(5):446-9 PMID: 4992452
The antibody response of mice to murine leukemia virus in spontaneous infection: absence of classical immunologic tolerance (AKR mice-complement-fixing antibodies-lymphocytic choriomeningitis virus-immunofluorescence-glomerular deposits of antigen-antibody complexes).
Proc Natl Acad Sci U S A. 1972 Jan;69(1):134-8 PMID: 4550501
A CONTRIBUTION TO THE QUESTION OF THE ANTIGENICITY OF SPONTANEOUS LYMPHOID AKR LEUKAEMIA.
Folia Biol (Praha). 1964;10:293-300 PMID: 14189806
Deficiency of the fifth component of complement in mice with an inherited complement defect.
J Exp Med. 1967 Jan 1;125(1):1-16 PMID: 4959665
Interferon and cell division. I. Inhibition of the multiplication of mouse leukemia L 1210 cells in vitro by interferon preparations.
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1052-8 PMID: 4319881
"Spontaneous" leukemia developing in C3H mice following inoculation in infancy, with AK-leukemic extracts, or AK-embrvos.
Proc Soc Exp Biol Med. 1951 Jan;76(1):27-32 PMID: 14816382
Inactivator of the third component of complement as an inhibitor in the properdin pathway.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2910-3 PMID: 4507613
Antigens of tumors and leukemias induced by viruses.
Fed Proc. 1965 Sep-Oct;24(5):1009-17 PMID: 5320101
Current enigmas in cancer research.
Harvey Lect. 1973;67:273-315 PMID: 4351100

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.138.4.925

Published

1973-10-01

Pages

925-38

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2180560

Subset

External Links

PubMed source DOI: 10.1084/jem.138.4.925 PMC Full Text View journal details

Analysis Services

Serum-mediated leukemia cell destruction in AKR mice.

Contact

WeChat Official Account