Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans.

Infection and immunity ·Vol. 24 ·No. 3 ·1979-06-00 ·Pages 865-8

St Martin EJ, Wittenberger CL

Abstract

A phosphoenolpyruvate-dependent sucrose phosphotransferase system has been identified in Streptococcus mutans. Sucrose phosphotransferase activity was inducible by sucrose and had an apparent Km for sucrose of 70 microM. The product of the sucrose phosphotransferase reaction was isolated and identified as sucrose phosphate. Additional analysis revealed that the phosphate group was on the glucose moiety. Mutants unable to grow in media containing low concentrations of sucrose were isolated and found to be missing either sucrose phosphotransferase activity or the ability to hydrolyze sucrose phosphate.

MeSH Terms

Enzyme Induction Kinetics Mutation Phosphoenolpyruvate/pharmacology Phosphotransferases/metabolism Streptococcus mutans/enzymology Sucrose/metabolism Sugar Phosphates/metabolism

Chemicals

Sugar Phosphates Sucrose Phosphoenolpyruvate Phosphotransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

St Martin E J

Wittenberger C L

References (14)

14 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.24.3.865-868.1979

Published

1979-06-00

Pages

865-8

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC414387

Subset

External Links

PubMed source DOI: 10.1128/iai.24.3.865-868.1979 PMC Full Text View journal details

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Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans.

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