Characterization of the DNA in Drosophila melanogaster.

Genetics ·Vol. 72 ·No. 3 ·1972-11-00 ·Pages 419-30

Travaglini EC, Petrovic J, Schultz J

Abstract

DNA has been quantitatively extracted from Drosophila melanogaster at various stages of embryonic development and analyzed by isopycnic centrifugation in CsCl and by fractionation on methylated albumin columns. The DNA is composed of three main classes of DNA, as defined by their buoyant density, rho, in CsCl: a bulk DNA, rho = 1.699 g cm(-3), and two satellite DNAs, rho = 1.685 g cm(-3) and rho = 1.669 g cm(-3). These three types of DNA persist throughout the development of the insect. In the unfertilized egg, 80% of the total DNA consists of the satellite DNAs; this amount decreases to 18% during the first three hours after fertilization and then remains constant through embryogenesis. There is a concomitant increase of the satellite DNA's with the bulk DNA after blastoderm formation.

MeSH Terms

Animals Centrifugation, Density Gradient Chromatography DNA/analysis Drosophila melanogaster Female Ovum/metabolism RNA/analysis

Chemicals

RNA DNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Travaglini E C

Petrovic J

Schultz J

References (14)

14 references, click to expand

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Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1093/genetics/72.3.419

Published

1972-11-00

Pages

419-30

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1212840

Subset

External Links

PubMed source DOI: 10.1093/genetics/72.3.419 PMC Full Text View journal details

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Characterization of the DNA in Drosophila melanogaster.

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