Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.

Nucleic acids research ·Vol. 6 ·No. 6 ·1979-00-00 ·Pages 2041-56

Potapov VK, Veiko VP, Koroleva ON, Shabarova ZA

Abstract

Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 mumol per gram of support.

MeSH Terms

Base Sequence Methods Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Polymers Spectrophotometry, Infrared

Chemicals

Oligodeoxyribonucleotides Oligonucleotides Polymers

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Potapov V K

Veiko V P

Koroleva O N

Shabarova Z A

References (9)

9 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.6.2041

Published

1979-00-00

Pages

2041-56

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327835

Subset

External Links

PubMed source DOI: 10.1093/nar/6.6.2041 PMC Full Text View journal details

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Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support.

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