Home LiteratureArticle Details
PMID: 4607609 Published · ppublish English Journal Article

Release of DNA in circulating blood and induction of anti-DNA antibodies after injection of bacterial lipopolysaccharides.

The Journal of experimental medicine ·Vol. 140 ·No. 5 ·1974-11-01 ·Pages 1189-206

Fournié GJ, Lambert PH, Meischer PA

Abstract

The present data demonstrate the induction of antisingle-stranded (SS) DNA and antidouble-stranded DNA antibodies in various strains of mice, including athymic C57BL/6 nude mice, after the injection of bacterial lipopolysaccharide (LPS). This anti-DNA response is dose dependent and varies quantitatively according to the strain of the injected mice. It is not correlated to the H-2 histocompatibility locus nor to the immune response to LPS. The lipid A fraction appears to be the active part of the LPS molecule for this particular effect. In addition, it was found that DNA is released in circulating blood a few hours after the injection of LPS. Most of the DNA released has physicochemical and immunochemical characteristics of SS DNA. Therefore, the anti-DNA response induced by injections of LPS may be the result of a release of DNA in a particularly immunogenic form at a time when the immune system, in particular the B lymphocytes, is rendered capable by LPS of developing an immune response to such a soluble antigen. These effects of LPS may account for the triggering or the exacerbation of ante-DNA antibodies during infections with gram-negative bacteria, and a similar mechanism may be involved in the pathogenesis of systemic lupus erythematosus.

MeSH Terms
Animals Antibodies, Antinuclear Antibody Formation Antibody Specificity Antigens, Bacterial Chromatography, Gel DNA/blood,immunology DNA, Single-Stranded/immunology Escherichia coli/immunology Hybridization, Genetic Immunization Lipopolysaccharides/immunology Lupus Erythematosus, Systemic/immunology Mice Mice, Inbred Strains Radioimmunoassay Salmonella enteritidis/immunology Salmonella typhimurium/immunology
Chemicals
Antibodies, Antinuclear Antigens, Bacterial DNA, Single-Stranded Lipopolysaccharides DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fournié G J
Lambert P H
Meischer P A
References (37)
37 references, click to expand
  1. The antigenic constituents of the neutrophilic leukocyte with special reference to the L. E. phenomenon.
    Vox Sang. 1957 Jul;2(3):145-58 PMID: 13468363
  2. Stimulation of B-lymphocytes by endotoxin. Reactions of thymus-deprived mice and karyotypic analysis of dividing cells in mice bearing T 6 T 6 thymus grafts.
    J Immunol. 1972 Apr;108(4):1088-91 PMID: 5023169
  3. The ability of bacterial lipopolysaccharide to modulate the induction of unresponsiveness to a state of immunity. Cellular parameters.
    J Exp Med. 1973 Dec 1;138(6):1481-95 PMID: 4128441
  4. Affinity between the lupus erythematosus serum factor and cell nuclei and nucleoprotein.
    Science. 1957 Jul 26;126(3265):162-3 PMID: 13442661
  5. The effect of endotoxin on tissue cultures of spleen of normal and tuberculin-sensitive animals.
    Am Rev Respir Dis. 1961 Dec;84:862-71 PMID: 13906006
  6. Functional characteristics of Peyer's patch lymphoid cells. II. Lipopolysaccharide is thymus dependent.
    J Exp Med. 1974 Feb 1;139(2):407-13 PMID: 4589992
  7. Mouse thymus-independent and thymus-derived lymphoid cells. I. Immunofluorescent and functional studies.
    J Exp Med. 1972 Nov 1;136(5):984-1007 PMID: 4117194
  8. Genesis of antinuclear antibody in NZB-W mice: role of genetic factors and of viral infections.
    Clin Exp Immunol. 1970 Jun;6(6):829-39 PMID: 4320133
  9. Further implication of murine leukemia-like virs in the disorders of NZB mice.
    J Exp Med. 1969 May 1;129(5):1045-62 PMID: 4305180
  10. The endotoxins of gram-negative bacteria and host resistance.
    N Engl J Med. 1961 May 4;264:919-23 PMID: 13743327
  11. DNA-binding assay for detection of anti-DNA antibodies in NZB-NZW F1 mice.
    J Immunol. 1969 Mar;102(3):788-90 PMID: 5304991
  12. Immunopathology of New Zealand black (NZB) mice.
    Transplantation. 1965 Nov;3(6):711-29 PMID: 4159831
  13. ANTINUCLEAR FACTOR IN MICE.
    J Immunol. 1964 Jul;93:148-54 PMID: 14214379
  14. Comparative study of antibodies to native and denatured DNA.
    J Immunol. 1970 Apr;104(4):902-6 PMID: 4986165
  15. Induction of immunoglobulin and antibody synthesis in vitro by lipopolysaccharides.
    Eur J Immunol. 1972 Aug;2(4):349-53 PMID: 4563347
  16. Recent observations and concepts in immunological unresponsiveness and autoimmunity.
    Clin Exp Immunol. 1971 Oct;9(4):437-47 PMID: 4107839
  17. Frequency of antigen-sensitive cells to thymus-independent antigens.
    Cell Immunol. 1971 Aug;2(4):309-16 PMID: 4942636
  18. The bacterial factor in traumatic shock.
    N Engl J Med. 1959 Jan 29;260(5):214-20 PMID: 13622952
  19. Pathogenesis of the glomerulonephritis of NZB/W mice.
    J Exp Med. 1968 Mar 1;127(3):507-22 PMID: 4169964
  20. Systemic lupus erythematosus: prototype of immune complex nephritis in man.
    J Exp Med. 1971 Sep 1;134(3):169-79 PMID: 19867364
  21. Termination of tolerance to human gamma globulin in mice by antigen and bacterial lipopolysaccharide (endotoxin).
    J Exp Med. 1973 Mar 1;137(3):740-50 PMID: 4120288
  22. The effect of induced chronic viral infections on the immunologic diseases of New Zealand mice.
    J Exp Med. 1970 Jul 1;132(1):89-109 PMID: 4323748
  23. A DNA-reacting factor in serum of a patient with lupus erythematosus diffusus.
    Proc Soc Exp Biol Med. 1957 Dec;96(3):572-4 PMID: 13505795
  24. Immune response of mice to nucleic acids: strain-dependent differences in magnitude and class of antibody production.
    J Immunol. 1973 Jul;111(1):121-9 PMID: 4576583
  25. The clearance and localization of nucleic acids by New Zealand and normal mice.
    Clin Exp Immunol. 1972 Dec;12(4):465-76 PMID: 4650369
  26. Iodination of nucleic acids in vitro.
    Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
  27. Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):2129-33 PMID: 4579014
  28. Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide.
    J Exp Med. 1956 Feb 1;103(2):225-46 PMID: 13286429
  29. The natural history of the NZB/NZW F1 hybrid mouse: a laboratory model of systemic lupus erythematosus.
    Australas Ann Med. 1965 Aug;14(3):185-91 PMID: 5862644
  30. The immunology and pathology of NZB mice.
    Adv Immunol. 1968;9:215-66 PMID: 4883742
  31. Activation of T and B lymphocytes in vitro. I. Regulatory influence of bacterial lipopolysaccharide (LPS) on specific T-cell helper function.
    J Exp Med. 1974 Jan 1;139(1):24-43 PMID: 4128447
  32. ANTI-DNA ANTIBODIES IN HYPERIMMUNIZED RABBITS.
    J Exp Med. 1965 Feb 1;121:309-21 PMID: 14264274
  33. Deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid.
    Science. 1968 Aug 23;161(3843):806-7 PMID: 4969617
  34. Rapidly induced changes in the level of non-specific immunity in laboratory animals.
    Br J Exp Pathol. 1956 Jun;37(3):223-34 PMID: 13342346
  35. Significance of anti-D.N.A. antibodies in systemic lupus erythematosus.
    Lancet. 1971 Oct 16;2(7729):861-3 PMID: 4106884
  36. Molecular immunogenicity of bacterial lipopolysaccharide antigens: establishing a quantitative system.
    J Immunol. 1971 Apr;106(4):993-1001 PMID: 4928750
  37. Mechanisms of tolerance and autoimmunity.
    Ann Rheum Dis. 1973 Jul;32(4):283-93 PMID: 4131672
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.140.5.1189
Published
1974-11-01
Pages
1189-206
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139721
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.140.5.1189 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2