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PMID: 4605075 Published · ppublish English Journal Article

Role of ATP in excision repair of ultraviolet radiation damage in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2651-4

Waldstein EA, Sharon R, Ben-Ishai R

Abstract

The effect of ATP on the first step of excision repair of ultraviolet damage in DNA has been studied using toluene-treated E. coli. During postirradiation incubation, five to six times more single-strand breaks are formed in DNA in the presence of exogenous ATP than in its absence. The ATP-dependent as well as the ATP-independent endonucleolytic activities appear to be catalyzed by the same enzyme since both activities are almost completely absent in uvrA and uvrB mutants. An ATP-dependent endonucleolytic activity has been detected in nonirradiated toluene-treated E. coli. It is concluded that ATP is required in vivo for either the incision step of repair or an enzymatic reaction preceding it.

MeSH Terms
Adenosine Triphosphate/physiology Centrifugation, Density Gradient DNA Repair/drug effects DNA, Bacterial/radiation effects DNA, Single-Stranded/radiation effects Endonucleases/metabolism Escherichia coli/drug effects,enzymology,metabolism Molecular Weight Mutation Radiation Dosage Radiation Effects Toluene/pharmacology Ultraviolet Rays
Chemicals
DNA, Bacterial DNA, Single-Stranded Toluene Adenosine Triphosphate Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Waldstein E A
Sharon R
Ben-Ishai R
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.7.2651
Published
1974-07-00
Pages
2651-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388524
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.7.2651 PMC Full Text View journal details
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