Home LiteratureArticle Details
PMID: 4596143 Published · ppublish English Journal Article

Isolation of nuclear acidic proteins from rat tissues. Characterization of acetylated liver nuclear acidic proteins.

The Biochemical journal ·Vol. 137 ·No. 2 ·1974-02-00 ·Pages 355-62

Suria D, Liew CC

Abstract

Nuclear acidic proteins isolated from rat brain, heart, kidney and liver showed similar, complex patterns on electrophoresis in sodium dodecyl sulphate-polyacrylamide gels. The contamination of nuclear acidic proteins by nuclear-membrane acidic proteins was found to the extent of 11%. Incorporation of [(3)H]acetate into the various nuclear acidic proteins in vivo, which were fractionated by polyacrylamide-gel electrophoresis, differed from tissue to tissue. Hydrolysis of these acetylated nuclear acidic proteins with 6m-HCl at 110 degrees C released 70% of the radioactivity, which indicated that labile acetyl groups had been incorporated into these proteins. Analysis of [(3)H]acetate-labelled nuclear acidic proteins revealed two acetylated amino acid residues, N(2)-acetylserine and N(2)-acetyl-lysine. The significance of the role played by nuclear acidic proteins in relation to gene regulation is discussed.

MeSH Terms
Acetates/metabolism Animals Brain/cytology Brain Chemistry Carbon Radioisotopes Cell Nucleus/analysis Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Hydrogen-Ion Concentration Kidney/analysis,cytology Liver/analysis,cytology Male Microscopy, Electron Microscopy, Phase-Contrast Myocardium/analysis,cytology Nerve Tissue Proteins/isolation & purification Nucleoproteins/isolation & purification Organ Specificity Protein Conformation Rats Tritium
Chemicals
Acetates Carbon Radioisotopes Nerve Tissue Proteins Nucleoproteins Tritium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suria D
Liew C C
References (33)
33 references, click to expand
  1. Solubilization and characterization of the residual proteins of the cell nucleus.
    J Biol Chem. 1966 Jun 25;241(12):2913-7 PMID: 5912366
  2. Phosphoprotein metabolism in isolated lymphocyte nuclei.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1182-9 PMID: 5225515
  3. The isolation, properties, and possible functions of chromatin acidic proteins.
    J Biol Chem. 1967 Mar 25;242(6):1220-6 PMID: 6024756
  4. Acidic proteins of mammalian nuclei: isolation and characterization.
    Proc Natl Acad Sci U S A. 1968 Jan;59(1):262-8 PMID: 4874832
  5. Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone.
    J Biol Chem. 1968 Oct 10;243(19):5018-22 PMID: 5679978
  6. Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.
    J Biol Chem. 1968 Dec 25;243(24):6361-6 PMID: 5726891
  7. Role of chromatin in estrogen action in the uterus. II. Hormone-induced synthesis of nonhistone acidic proteins which restore histone-inhibited DNA-dependent RNA synthesis.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):465-72 PMID: 5257138
  8. Isolation, morphology, and composition of the nuclear membrane from rat liver.
    J Biol Chem. 1969 Jul 25;244(14):3786-92 PMID: 4308735
  9. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  10. Selective synthesis of a nuclear acidic protein in liver cells stimulated by cortisol.
    Nature. 1970 Oct 10;228(5267):132-4 PMID: 5460012
  11. Limited heterogeneity of the major nonhistone chromosomal proteins.
    Biochemistry. 1970 Oct 27;9(22):4440-7 PMID: 5472713
  12. DNA synthesis associated with the inner membrane of the nuclear envelope.
    Nat New Biol. 1971 Jan 6;229(1):22-4 PMID: 5276050
  13. Activated transcription from rat liver chromatin by non-histone proteins.
    Biochim Biophys Acta. 1971 Jan 28;228(2):563-76 PMID: 5545883
  14. Studies of nuclear acidic proteins. Evidence for their phosphorylation, tissue specificity, selective binding to deoxyribonucleic acid, and stimulation effects on transcription.
    J Biol Chem. 1971 Jun 10;246(11):3597-609 PMID: 4397103
  15. The effect of cell-sap fractions on incorporation of amino acids into protein in rat liver cell-free systems.
    Can J Biochem. 1970 Jul;48(7):736-9 PMID: 5512547
  16. Organ-specific restriction of transcription in mammalian chromatin.
    J Mol Biol. 1968 Jul 14;34(2):305-16 PMID: 5761390
  17. Nuclear residual proteins from goose erythroid cells and liver.
    Biochemistry. 1971 Jun 8;10(12):2342-8 PMID: 5114992
  18. Effects of aldosterone on RNA polymerase in rat heart and kidney nuclei.
    Endocrinology. 1972 Feb;90(2):488-95 PMID: 4550536
  19. 50-S ribosomal proteins. Peptide studies on two acidic proteins, A 1 and A 2 , isolated from 50-S ribosomes of Escherichia coli.
    Eur J Biochem. 1972 Jan 31;25(1):13-9 PMID: 4554103
  20. Membrane phospholipids associated with nuclei and chromatin: melting profile, template activity and stability of chromatin.
    J Mol Biol. 1972 Jun 20;67(2):231-46 PMID: 4557202
  21. Mechanism of action of gonadotropin. I. Evidence for gonadotropin-induced modifications of ovarian nuclear basic and acidic protein biosynthesis, phosphorylation, and acetylation.
    J Biol Chem. 1972 Sep 10;247(17):5535-42 PMID: 5055781
  22. The non-histone proteins of chromatin, their isolation and composition in a number of tissues.
    Biochim Biophys Acta. 1972 Aug 25;277(2):384-402 PMID: 5075291
  23. The basic proteins of trout testis chromatin: aspects of their synthesis, post-synthetic modifications and binding to DNA.
    Acta Endocrinol Suppl (Copenh). 1972;168:130-54 PMID: 4562651
  24. A method for the rapid isolation of nuclear membranes from rat liver. Characterisation of the membrane preparation and its associated DNA polymerase.
    Eur J Biochem. 1972 Oct 17;30(1):145-54 PMID: 4343806
  25. Differential effects of cyclic adenosine-3',5'-monophosphate on phosphorylation of rat liver nuclear acidic proteins.
    Arch Biochem Biophys. 1972 Oct;152(2):786-94 PMID: 4344132
  26. Kinetics of enzymatic modification of the protamines and a proposal for their binding to chromatin.
    J Biol Chem. 1972 Dec 25;247(24):7962-8 PMID: 4640934
  27. Acetylation of ribosomal proteins. I. Characterization and properties of rat liver ribosomal proteins.
    J Biol Chem. 1973 Feb 10;248(3):977-83 PMID: 4684719
  28. Histone acetylation and hormone action. Early effects of oestradiol-17beta on histone acetylation in rat uterus.
    Biochem J. 1972 Dec;130(3):663-9 PMID: 4664926
  29. Effects of aldosterone on acetylation and phosphorylation of chromosomal proteins.
    Endocrinology. 1973 Nov;93(5):1025-34 PMID: 4778752
  30. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  31. Phosphorus assay in column chromatography.
    J Biol Chem. 1959 Mar;234(3):466-8 PMID: 13641241
  32. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
  33. A new reagent for detection of peptides, nucleotides, and other N-H-containing compounds on paper chromatograms.
    J Biol Chem. 1962 May;237:1619-21 PMID: 14471820
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1370355
Published
1974-02-00
Pages
355-62
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166124
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1370355 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2