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PMID: 4664926 Published · ppublish English Journal Article

Histone acetylation and hormone action. Early effects of oestradiol-17beta on histone acetylation in rat uterus.

The Biochemical journal ·Vol. 130 ·No. 3 ·1972-12-00 ·Pages 663-9

Libby PR

Abstract

The effect of oestradiol treatment on the acetylation of histones of the immature rat uterus has been studied. A 10mug dose of oestradiol causes a 70% increase at 5min and a 140% increase at 10min after administration in the labelling of the histone fraction F2+F3. No effect of oestradiol is seen on the labelling of histones F1 or acidic non-histone chromatin proteins. The oestradiol stimulation is seen in animals pretreated with either cycloheximide or actinomycin D. The stimulation of labelling caused by oestradiol is completely abolished by pretreatment of the animals with the anti-oestrogen, nafoxidine. The stimulation is given by lower doses of oestradiol, by stilboestrol and oestriol, but is not given by testosterone. These results suggest that stimulation of histone acetylation in the uterus is the earliest known effect of the hormone on its target tissue.

MeSH Terms
Acetates/metabolism Acetylation Animals Anti-Bacterial Agents/pharmacology Chromatin/metabolism Cycloheximide/pharmacology Dactinomycin/pharmacology Diethylstilbestrol/pharmacology Estradiol/metabolism,pharmacology Estriol/pharmacology Estrogen Antagonists Female Histones/metabolism Liver/metabolism Naphthalenes/pharmacology Phenols/pharmacology Pyrrolidines/pharmacology Rats Stimulation, Chemical Testosterone/pharmacology Tritium Uterus/drug effects,metabolism
Chemicals
Acetates Anti-Bacterial Agents Chromatin Estrogen Antagonists Histones Naphthalenes Phenols Pyrrolidines Tritium Dactinomycin Testosterone Estradiol Diethylstilbestrol Cycloheximide Estriol
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Libby P R
References (17)
17 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1300663
Published
1972-12-00
Pages
663-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1174504
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1300663 PMC Full Text View journal details
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