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PMID: 4588271 Published · ppublish English Comparative Study Journal Article

Chemical characterization of the isolated cell surface of Amoeba.

The Journal of cell biology ·Vol. 60 ·No. 1 ·1974-01-00 ·Pages 26-38

Allen HJ, Ault C, Winzler RJ, Danielli JF

Abstract

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.

MeSH Terms
Acid Phosphatase/analysis Amino Acids/analysis Amoeba/analysis,cytology Animals Calcium/analysis Cell Membrane/analysis Centrifugation, Density Gradient Chromatography, Gel Glycoside Hydrolases/analysis Hexosamines/analysis Histocytochemistry Lipids/analysis Microscopy, Electron Microscopy, Phase-Contrast Monosaccharides/analysis Neuraminic Acids/analysis Phosphates/analysis Pronase Proteins/analysis RNA/analysis Species Specificity Succinate Dehydrogenase/analysis
Chemicals
Amino Acids Hexosamines Lipids Monosaccharides Neuraminic Acids Phosphates Proteins RNA Succinate Dehydrogenase Acid Phosphatase Glycoside Hydrolases Pronase Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Allen H J
Ault C
Winzler R J
Danielli J F
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.60.1.26
Published
1974-01-00
Pages
26-38
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109129
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.60.1.26 PMC Full Text View journal details
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