Abstract
Mutations in capR or capT cause derepression of the enzymes of the gal operon. The gal-specific messenger ribonucleic acid is directly proportional to the gal enzyme levels in wild type, capR, and capT strains. These results indicate that capR and capT control the gal operon at the transcriptional level.
MeSH Terms
Cell-Free System
DNA, Bacterial
Escherichia coli/enzymology,metabolism
Galactose/metabolism
Genes
Isomerases/metabolism
Mutation
Nucleic Acid Hybridization
Operon
Phosphotransferases/metabolism
RNA, Bacterial/biosynthesis
RNA, Messenger/biosynthesis
Transcription, Genetic
Tritium
Chemicals
DNA, Bacterial
RNA, Bacterial
RNA, Messenger
Tritium
Phosphotransferases
Isomerases
Galactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Buchanan C E
Hua S S
Avni H
Markovitz A
References (14)
14 references, click to expand
-
Synthesis and turnover of phage messenger RNA in E. coli infected with bacteriophage T4 in the presence of chloromycetin.
J Mol Biol. 1962 Nov;5:527-34
PMID: 13939775
-
Multiple regulator gene control of the galactose operon in Escherichia coli K-12.
J Bacteriol. 1972 Jun;110(3):1089-99
PMID: 4555404
-
The galactose operon of E. coli K-12. II. A deletion analysis of operon structure and polarity.
Genetics. 1969 Jun;62(2):249-64
PMID: 4905872
-
Isolation of the gal repressor.
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1891-5
PMID: 4942917
-
[REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. II. THE GENETIC DETERMINISM OF THE REGULATION].
J Mol Biol. 1963 Aug;7:183-205
PMID: 14062651
-
HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.
Proc Natl Acad Sci U S A. 1959 Dec;45(12):1776-86
PMID: 16590575
-
Regulation of lac messenger ribonucleic acid synthesis by cyclic adenosine 3',5'-monophosphate and glucose.
J Biol Chem. 1970 May 10;245(9):2259-67
PMID: 4315149
-
Regulation of gal messenger ribonucleic acid synthesis in Escherichia coli by 3',5'-cyclic adenosine monophosphate.
J Biol Chem. 1971 May 10;246(9):2898-903
PMID: 4324343
-
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
J Mol Biol. 1965 Jul;12(3):829-42
PMID: 4955314
-
Isolation of plaque-forming, galactose-transducing strains of phage lambda.
Genetics. 1972 Jun;71(2):189-206
PMID: 4558323
-
Orientation of transcription for the galactose operon as determined by hybridization of gal mRNA with the separated DNA strands of coliphages lambda-dg.
J Mol Biol. 1968 Jul 14;35(1):207-13
PMID: 4939778
-
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75
PMID: 14907713
-
Negative control of the galactose operon in E. coli.
Mol Gen Genet. 1968;102(1):79-88
PMID: 4883532
-
Regulation of the gal operon of Escherichia coli by the capR gene.
J Biol Chem. 1972 May 25;247(10):2973-8
PMID: 4337501