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PMID: 4547437 Published · ppublish English Journal Article

Evidence for the expression of Ia (H-2-associated) antigens on thymus-derived lymphocytes.

The Journal of experimental medicine ·Vol. 140 ·No. 5 ·1974-11-01 ·Pages 1273-84

Frelinger JA, Neiderhuber JE, David CS, Shreffler DC

Abstract

We have demonstrated in an anti-Ia serum the presence of specific antibodies reacting with T cells, as well as with B cells, using a highly sensitive dye exclusion test. This antiserum reacts with both spleen and lymph node in a characteristic biphasic titration curve killing up to 70% of these cells. It also reacts with cortisone-resistant thymocytes. The A.TH-alpha-A.TL serum can be absorbed with spleen, lymph node, cortisone-resistant thymus, or normal thymus cells. Further in vivo absorptions in BALB/c nude cannot remove all of the cytotoxic activity for normal BALB lymph node lymphocytes, while completely removing the activity for nude cells. A Thy-1 positive cell line derived from a C57Br leukemia is reactive with this anti-Ia serum.

MeSH Terms
Animals Antibody Formation Antibody Specificity Antilymphocyte Serum Cortisone/pharmacology Cytotoxicity Tests, Immunologic Erythrocytes/immunology Genes Histocompatibility Antigens Lymph Nodes/cytology Mice Mice, Inbred Strains/immunology Rabbits/immunology Sheep/immunology Spleen/cytology T-Lymphocytes/drug effects,immunology
Chemicals
Antilymphocyte Serum Histocompatibility Antigens Cortisone
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Frelinger J A
Neiderhuber J E
David C S
Shreffler D C
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24 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.140.5.1273
Published
1974-11-01
Pages
1273-84
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139718
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.140.5.1273 PMC Full Text View journal details
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