Differentiation of cell membranes in cultures of embryonic chick breast muscle.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3208-11

Prives JM, Paterson BM

Abstract

Three components of differentiated muscle membrane, the acetylcholine receptor, acetylcholinesterase (EC 3.1.1.7; acetylcholine hydrolase), and adenylate cyclase [EC 4.6.1.1; ATP pyrophosphate-lyase (cyclizing)], appear simultaneously during myogenesis in cultures of embryonic chick muscle, after the main period of rapid cell fusion. However, unlike the cytoplasmic proteins of differentiated muscle, the elaboration of these membrane components is unaltered when fusion is blocked by lowering the calcium concentration in the medium. These results suggest that membrane differentiation and cytoplasmic differentiation are regulated independently during muscle development.

MeSH Terms

Acetylcholinesterase/metabolism Adenylyl Cyclases/metabolism Animals Bungarotoxins/metabolism Calcium/pharmacology Cell Differentiation Cell Fusion/drug effects Cell Membrane/enzymology Chick Embryo Culture Techniques Cytoplasm/enzymology Iodine Radioisotopes Kinetics Muscles/embryology,enzymology Receptors, Cholinergic

Chemicals

Bungarotoxins Iodine Radioisotopes Receptors, Cholinergic Acetylcholinesterase Adenylyl Cyclases Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Prives J M

Paterson B M

References (21)

21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.8.3208

Published

1974-08-00

Pages

3208-11

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388652

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.8.3208 PMC Full Text View journal details

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Differentiation of cell membranes in cultures of embryonic chick breast muscle.

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