The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 7 ·1974-07-00 ·Pages 2863-6

Beuzard Y, London IM

Abstract

Protein synthesis in reticulocyte lysates ceases abruptly in the absence of added hemin or in the presence of double-stranded RNA. A similar effect of double-stranded RNA is observed in Krebs II ascites cell-free systems translating exogenous globin mRNA. The shut-off of protein synthesis is due to inhibition of initiation and can be prevented or reversed by addition of the initiation factor preparation M(3). Preparations of M(1), M(2), and dissociation factor are ineffective under these conditions. The effects of added hemin, M(3), and globin mRNA on the synthesis of alpha and beta globin chains have been studied in the reticulocyte and ascites cell extracts. When the concentration of M(3) is rate limiting, the synthesis of beta chains exceeds that of alpha chains. When the concentration of mRNA is rate limiting, synthesis of alpha and beta chains is more nearly equal.

MeSH Terms

Animals Carcinoma, Krebs 2/metabolism Cell-Free System Globins/biosynthesis Heme/pharmacology Peptide Chain Initiation, Translational Peptide Initiation Factors/pharmacology Protein Biosynthesis RNA/pharmacology RNA, Messenger Rabbits Reticulocytes/metabolism Tritium

Chemicals

Peptide Initiation Factors RNA, Messenger Tritium Heme RNA Globins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Beuzard Y

London I M

References (29)

29 references, click to expand

The stimulation of globin synthesis by heme.
Proc Natl Acad Sci U S A. 1966 Mar;55(3):650-5 PMID: 5221248
Hemoglobin synthesis during amphibian metamorphosis. I. Chemical studies on the hemoglobins from the larval and adult stages of Rana catesbeiana.
J Mol Biol. 1968 Mar 28;32(3):481-92 PMID: 5644917
Stimulation of globin-chain initiation by hemin in the reticulocyte cell-free system.
Proc Natl Acad Sci U S A. 1968 Feb;59(2):582-9 PMID: 5238986
Factors affecting the rate of protein synthesis in lysate systems from reticulocytes.
Arch Biochem Biophys. 1968 May;125(2):671-83 PMID: 5656815
The role of heme in the synthesis and assembly of hemoglobin.
J Biol Chem. 1968 Oct 10;243(19):4987-99 PMID: 5679976
Translational control in hemoglobin syntheskis.
Cold Spring Harb Symp Quant Biol. 1969;34:567-78 PMID: 5266178
Studies on cessation of protein synthesis in a reticulocyte lysate cell-free system.
Biochim Biophys Acta. 1970 Jul 16;213(1):237-40 PMID: 5488930
Cytoplasm from poliovirus-infected HeLa cells inhibits cell-free haemoglobin synthesis.
Nat New Biol. 1971 Mar 17;230(11):91-4 PMID: 4323460
Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2752-6 PMID: 5288252
The control of hemoglobin synthesis. A comparison of the role of heme in rabbit bone marrow and reticulocytes.
J Biol Chem. 1972 Jan 25;247(2):326-33 PMID: 5009688
Myosin synthesis and specificity of eukaryotic initiation factors.
Biochemistry. 1972 May 23;11(11):2061-6 PMID: 5027616
Control of globin synthesis in cell-free preparations of reticulocytes by formation of a translational repressor that is inactivated by hemin.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1565-8 PMID: 4504369
Separation and translation of the mRNAs coding for and chains of rabbit globin.
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1574-7 PMID: 4504371
Control of globin synthesis: the role of heme.
J Mol Biol. 1972 May 28;66(3):471-81 PMID: 5037023
Further studies on the translation of globin mRNA and encephalomyocarditis virus RNA in a cell-free system from Krebs II ascites cells.
Biochim Biophys Acta. 1972 Jun 22;272(1):108-18 PMID: 4339672
A complex between met-tRNA F and native 40S subunits in reticulocyte lysates and its disappearance during incubation with double-stranded RNA.
Biochem Biophys Res Commun. 1972 Sep 26;48(6):1556-64 PMID: 5077836
Involvement of hemin, a stimulatory fraction from ribosomes and a protein synthesis inhibitor in the regulation of hemoglobin synthesis.
J Mol Biol. 1972 Jan 28;63(2):247-64 PMID: 4634507
Control of protein synthesis in reticulocyte lysates by haemin.
Nat New Biol. 1973 Jan 31;241(109):150-2 PMID: 4512619
Specificity in initiation of protein synthesis in a fractionated mammalian cell-free system.
Nat New Biol. 1973 Apr 4;242(118):136-40 PMID: 4349564
Biosynthesis of reticulocyte membrane proteins by membrane-free polyribosomes.
Proc Natl Acad Sci U S A. 1973 May;70(5):1526-30 PMID: 4514321
Specificity of the control of protein synthesis by haemin.
Nat New Biol. 1973 Jun 20;243(129):230-3 PMID: 4351984
Effect of hemin on the synthesis of hemoglobin and other proteins in mammalian cells.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1022-6 PMID: 4197926
Inhibition of cellular protein synthesis by double-stranded RNA: inactivation of an initiation factor.
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1222-6 PMID: 4197930
Discrimination between messenger ribonucleic acids by a mammalian translation initiation factor.
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2139-44 PMID: 4352974
Presence of a new RNA species among the initiation protein factors active in eukaryotes translation.
Biochem Biophys Res Commun. 1973 Sep 5;54(1):25-32 PMID: 4741566
Differential translation of alpha- and beta-globin messenger in a cell-free system.
FEBS Lett. 1973 Sep 1;35(1):45-50 PMID: 4751998
Letter: Haem deficiency and chain synthesis.
Nature. 1974 Mar 1;248(5443):88 PMID: 4818567
THE EFFECT OF HEMIN ON THE SYNTHESIS OF GLOBIN.
Biochem Biophys Res Commun. 1965 Jan 18;18:236-42 PMID: 14282023
Translational control of and globin chain synthesis.
Nat New Biol. 1972 Dec 27;240(104):274-6 PMID: 4512231

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.7.2863

Published

1974-07-00

Pages

2863-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388572

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.7.2863 PMC Full Text View journal details

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The effects of hemin and double-stranded RNA on alpha and beta globin synthesis in reticulocyte and Krebs II ascites cell-free systems and the relationship of these effects to an initiation factor preparation.

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