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PMID: 4508333 Published · ppublish English Journal Article

Induction of auxotrophic mutations by treatment of Chinese hamster cells with 5-bromodeoxyuridine and black light.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 11 ·1972-11-00 ·Pages 3459-63

Chu EH, Sun NC, Chang CC

Abstract

Treatment of an aneuploid Chinese hamster cell line (V79) in tissue culture with 5-bromodeoxyuridine and black light, with or without prior exposure of cells to another mutagen, led to the isolation of auxotrophic mutants, each of which exhibits a specific nutritional requirement for glycine, uridine, purine, or for a combination of glycine, hypoxanthine, and thymidine. 61 mutants that could not use exogenous galactose were also isolated. Thus, this treatment functions as an inductive, but not as a selective, procedure for mutations in mammalian cells.

MeSH Terms
Animals Azaguanine/pharmacology Bromodeoxyuridine/pharmacology Cell Line Cell Survival Cricetinae Culture Media Fluorescence Galactose/metabolism Glucose/metabolism Glycine/metabolism Hypoxanthines/metabolism Mutagens Mutation/drug effects Thymidine/metabolism Uridine/metabolism
Chemicals
Culture Media Hypoxanthines Mutagens Bromodeoxyuridine Glucose Azaguanine Glycine Thymidine Uridine Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chu E H
Sun N C
Chang C C
References (20)
20 references, click to expand
  1. Effects of ultraviolet radiation on mammalian cells. I. Induction of chromosome aberrations.
    Mutat Res. 1965 Feb;2(1):75-94 PMID: 5896361
  2. Genetics of somatic mammalian cells. IV. Properties of Chinese hamster cell mutants with respect to the requirement for proline.
    Genetics. 1967 Mar;55(3):513-24 PMID: 6068403
  3. Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1227-34 PMID: 5233843
  4. Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
    Proc Natl Acad Sci U S A. 1968 Aug;60(4):1275-81 PMID: 5244736
  5. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1306-12 PMID: 5249812
  6. Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents.
    J Cell Physiol. 1969 Dec;74(3):245-58 PMID: 5390857
  7. DNA replication during episome transfer as studied by bromodeoxyuridine photosensitization.
    J Mol Biol. 1969 Dec 14;46(2):355-8 PMID: 4902521
  8. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro.
    Genetics. 1969 Jun;62(2):359-77 PMID: 5392645
  9. New class of purine mutants of Chinese hamster ovary cells.
    Science. 1971 Apr 9;172(3979):162-3 PMID: 5547727
  10. Mammalian cell genetics. 3. Characterization of x-ray-induced forward mutations in Chinese hamster cell cultures.
    Mutat Res. 1971 Jan;11(1):23-34 PMID: 5104862
  11. Genetics of somatic mammalian cells. XII. Mutagenesis by carcinogenic nitroso compounds.
    J Cell Physiol. 1971 Aug;78(1):139-44 PMID: 5165083
  12. The mutagenicity to mammalian cells of pyrimidine nucleoside analogs.
    Mutat Res. 1972 Jan;14(1):130-2 PMID: 4258180
  13. Mutagenic and lethal effects of visible and near-ultraviolet light on bacterial cells.
    Adv Genet. 1971;16:167-98 PMID: 4947103
  14. Survival response of asynchronous and synchronous Chinese hamster cells exposed to fluorescent light following 5-bromodeoxyuridine incorporation.
    Mutat Res. 1972 Feb;14(2):237-45 PMID: 5062702
  15. Plaque formation and isolation of pure lines with poliomyelitis viruses.
    J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
  16. Amino acid metabolism in mammalian cell cultures.
    Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
  17. A method of selecting for auxotrophic mutants of HeLa cells.
    J Exp Med. 1960 Apr 1;111:559-72 PMID: 13720702
  18. Genetics of human cell lines. III. Incorporation of 5-bromo- and 5-iododeoxyuridine into the deoxyribonucleic acid of human cells and its effect on radiation sensitivity.
    J Exp Med. 1960 Sep 1;112:509-31 PMID: 13723177
  19. A small and inexpensive ultraviolet dose-rate meter useful in biological experiements.
    Radiat Res. 1961 Apr;14:394-403 PMID: 13789181
  20. The action spectra for ultravioletlight inactivation of systems containing 5-bromouracil-substituted deoxyribonucleic acid. I. Escherichia coli 15 T A U.
    Biochim Biophys Acta. 1963 Mar 26;68:446-54 PMID: 14040806
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.11.3459
Published
1972-11-00
Pages
3459-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389793
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.11.3459 PMC Full Text View journal details
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