New class of purine mutants of Chinese hamster ovary cells.

Science (New York, N.Y.) ·Vol. 172 ·No. 3979 ·1971-04-09 ·Pages 162-3

Taylor MW, Souhrada M, McCall J

Abstract

Mutants of Chinese hamster ovary cells (CHO/Pro(-)) were isolated after mutagenesis with N-methylnitrosoguanidine and selection by the bromodeoxyuridine technique. Six of these were mutants classified as purine-requiring. The metabolic block appears to be early in the purine biosynthetic pathway. The mutants do not appear to be genetically identical.

MeSH Terms

Animals Bromodeoxyuridine/metabolism Cricetinae Culture Media Cytogenetics Diploidy Female Guanidines/pharmacology Humans Imidazoles/metabolism In Vitro Techniques Mutagens Mutation Nitroso Compounds/pharmacology Ovary/metabolism Purine-Pyrimidine Metabolism, Inborn Errors Purines/metabolism Transferases/metabolism

Chemicals

Culture Media Guanidines Imidazoles Mutagens Nitroso Compounds Purines Transferases Bromodeoxyuridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Taylor M W

Souhrada M

McCall J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.172.3979.162

Published

1971-04-09

Pages

162-3

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.172.3979.162 View journal details

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