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PMID: 4504337 Published · ppublish English Journal Article

Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1244-8

Sato K, Slesinski RS, Littlefield JW

Abstract

The presence of selectable genetic markers in long-term human lymphoblast cultures would facilitate cell hybridization experiments on the biosynthesis of immunoglobulins, as well as other studies. This work reports the induction with ethylmethane sulfonate of 6-thioguanine - resistant, phosphoribosyltransferase - deficient mutants in a lymphoblast line from a patient with infectious mononucleosis. These cells were unusually sensitive, with a D(0) value of 28 mug of ethylmethane sulfonate per ml; the sensitivity curve followed a biphasic pattern suggesting the presence of 3% resistant cells. Ethylmethane sulfonate increased the frequency of mutants resistant to 6-thioguanine over 100-fold, to about 2 x 10(-4); nitrosoguanidine was less effective. Almost all the mutants contained considerably less than 1% of the hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) activity of wild-type cells. The mutation did not appear to result from loss of an X chromosome.

MeSH Terms
Alkanes/pharmacology Cell Line Cells, Cultured/metabolism Chromosome Aberrations Female Gene Frequency Genetic Code Humans Hybridization, Genetic Infectious Mononucleosis Inosine Nucleotides Lesch-Nyhan Syndrome Lymphocytes/drug effects,enzymology Mutation Nitrosoguanidines/pharmacology Pentosephosphates Pentosyltransferases/metabolism Selection, Genetic Sulfonic Acids/pharmacology Thioguanine/pharmacology
Chemicals
Alkanes Inosine Nucleotides Nitrosoguanidines Pentosephosphates Sulfonic Acids Pentosyltransferases Thioguanine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sato K
Slesinski R S
Littlefield J W
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.5.1244
Published
1972-05-00
Pages
1244-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426673
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.5.1244 PMC Full Text View journal details
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