Abstract
A method was devised for obtaining single cell clones of human leukocytic cell lines in the presence of a human placental cell feeder layer. Clones of two lines, LS-B and EB(3), which contain Epstein-Barr viral (EBV) antigen in approximately 1% of cells were tested for EBV antigen. Since all EB(3) clones and LS-B clones contained EBV antigen, it is concluded that in vitro EBV genome is associated with all of the cells.
MeSH Terms
Antigens/analysis
Cell Line
Clone Cells
Culture Media
Culture Techniques
Fluorescent Antibody Technique
Genetics, Microbial
Herpesviridae/immunology
Herpesvirus 4, Human/immunology,pathogenicity
Humans
Immune Sera
Leukocytes
Chemicals
Antigens
Culture Media
Immune Sera
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller M H
Stitt D
Miller G
References (5)
5 references, click to expand
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