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PMID: 4320703 Published · ppublish English Journal Article

Epstein-Barr viral antigen in single cell clones of two human leukocytic lines.

Journal of virology ·Vol. 6 ·No. 5 ·1970-11-00 ·Pages 699-701

Miller MH, Stitt D, Miller G

Abstract

A method was devised for obtaining single cell clones of human leukocytic cell lines in the presence of a human placental cell feeder layer. Clones of two lines, LS-B and EB(3), which contain Epstein-Barr viral (EBV) antigen in approximately 1% of cells were tested for EBV antigen. Since all EB(3) clones and LS-B clones contained EBV antigen, it is concluded that in vitro EBV genome is associated with all of the cells.

MeSH Terms
Antigens/analysis Cell Line Clone Cells Culture Media Culture Techniques Fluorescent Antibody Technique Genetics, Microbial Herpesviridae/immunology Herpesvirus 4, Human/immunology,pathogenicity Humans Immune Sera Leukocytes
Chemicals
Antigens Culture Media Immune Sera
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Miller M H
Stitt D
Miller G
References (5)
5 references, click to expand
  1. Immunofluorescence in cells derived from Burkitt's lymphoma.
    J Bacteriol. 1966 Mar;91(3):1248-56 PMID: 4160230
  2. Studies with Burkitt's lymphoma.
    Wistar Inst Symp Monogr. 1965 Sep;4:69-82 PMID: 5884338
  3. Specific immunofluorescence test for the herpes-type EB virus of Burkitt lymphoblasts, authenticated by electron microscopy.
    J Natl Cancer Inst. 1968 Mar;40(3):593-607 PMID: 4868586
  4. Establishment of lines from normal human blood leukocytes by co-cultivation with a leukocyte line derived from a leukemic child.
    Proc Soc Exp Biol Med. 1969 Oct;132(1):247-52 PMID: 4899209
  5. Trypsinized placental cell cultures for the propagation of viruses and as "feeder layers".
    J Virol. 1970 Mar;5(3):406-9 PMID: 4314557
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1970-11-00
Pages
699-701
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376178
Subset
IM
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