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PMID: 4464846 Published · ppublish English Journal Article

Influence of ascorbic acid on ribosomal patterns and collagen biosynthesis in healing wounds of scorbutic guinea pigs.

The Biochemical journal ·Vol. 142 ·No. 3 ·1974-09-00 ·Pages 641-51

Harwood R, Grant ME, Jackson DS

Abstract

Scorbutic guinea pigs were wounded and the influence of administering ascorbic acid 6 days later was studied with respect to cellular morphology, ribosomal distribution and protein synthesis. Electron-microscopic studies revealed that the dilated endoplasmic reticulum observed in the fibroblasts of scorbutic wound tissue had reverted to a normal configuration 24h after intraperitoneal injection of 100mg of ascorbate. Quantitative determination of the distribution of free and membrane-bound ribosomes indicated a significant increase in membrane-bound ribosomes in wound tissue from ascorbate-supplemented (recovery) animals. Sucrose-density-gradient centrifugation indicated a significant increase in the proportion of large membrane-bound polyribosomes in the range 300-350S and a concomitant decrease in 80S monoribosomes in the ribosome sedimentation profile of recovery tissue. Determination of the synthesis of non-diffusible [(3)H]hydroxyproline in scorbutic and recovery wounds showed a 3-4-fold stimulation in peptidyl-proline hydroxylation in recovery tissues. Studies carried out in which scorbutic and recovery tissues were incubated with [(14)C]leucine indicated that general protein synthesis, as measured by (14)C incorporated into non-diffusible material/mug of DNA, was unaltered by ascorbate supplementation. Similar studies of [(3)H]proline incorporation suggested that in recovery tissues there was a small but significant increase in [(3)H]proline incorporated/mug of DNA, which probably represents an increase in protocollagen synthesis. This observation correlates well with the increase seen in recovery tissues of large polyribosomes on which collagen precursor polypeptides are known to be synthesized. Preliminary characterization of the repair collagen synthesized by recovery animals showed it to be a typical Type I collagen having the chain composition (alpha(1))(2)alpha(2). The extent of glycosylation of the hydroxylysine of the newly synthesized collagen was greater than that reported for either normal guinea-pig dermal collagen or dermal scar collagen.

MeSH Terms
Animals Ascorbic Acid/pharmacology Centrifugation, Density Gradient Collagen/biosynthesis Guinea Pigs Hydroxylysine/analysis Lysine/analysis Male Microscopy, Electron Polyribosomes/metabolism,ultrastructure Proline/metabolism Ribosomes/metabolism,ultrastructure Scurvy/metabolism,pathology Skin/metabolism,pathology,ultrastructure Wound Healing/drug effects
Chemicals
Hydroxylysine Collagen Proline Lysine Ascorbic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Harwood R
Grant M E
Jackson D S
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55 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1420641
Published
1974-09-00
Pages
641-51
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168330
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1420641 PMC Full Text View journal details
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