DNA uptake during genetic transformation and the growing zone of the cell envelope.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1848-52

Tomasz A, Zanati E, Ziegler R

Abstract

Continuous cellular incorporation of choline molecules is essential for DNA binding to competent pneumococci. The choline molecules are incorporated into the cell surface at the equatorial region of the cocci. As a working hypothesis, it is proposed that DNA molecules enter these bacteria at the growing zone(s) of the cell envelope.

MeSH Terms

Amino Alcohols/metabolism Autoradiography Bacteriolysis Binding Sites Cell Wall/metabolism Choline/metabolism Culture Media DNA, Bacterial/metabolism Microscopy, Electron Models, Biological Receptors, Drug Streptococcus pneumoniae/cytology,growth & development,metabolism Teichoic Acids/biosynthesis Thymidine/metabolism Transformation, Genetic Tritium

Chemicals

Amino Alcohols Culture Media DNA, Bacterial Receptors, Drug Teichoic Acids Tritium Choline Thymidine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Tomasz A

Zanati E

Ziegler R

References (14)

14 references, click to expand

Binding of the competence factor to receptors in the spheroplast membrane of pneumococci.
Biochem Biophys Res Commun. 1970 Dec 9;41(5):1342-9 PMID: 4394905
REGULATION OF THE TRANSFORMABILITY OF PHEUMOCOCCAL CULTURES BY MACROMOLECULAR CELL PRODUCTS.
Proc Natl Acad Sci U S A. 1964 Mar;51:480-7 PMID: 14171462
Nutritional Requirements of the Pneumococcus: I. Growth Factors for Types I, II, V, VII, VIII.
J Bacteriol. 1940 Nov;40(5):695-704 PMID: 16560380
Cellular metabolism in genetic transformation of pneumococci: requirement for protein synthesis during induction of competence.
J Bacteriol. 1970 Mar;101(3):860-71 PMID: 4392399
Biological consequences of the replacement of choline by ethanolamine in the cell wall of Pneumococcus: chanin formation, loss of transformability, and loss of autolysis.
Proc Natl Acad Sci U S A. 1968 Jan;59(1):86-93 PMID: 4385544
Multiple antibiotic resistance in a bacterium with suppressed autolytic system.
Nature. 1970 Jul 11;227(5254):138-40 PMID: 4393335
An autoradiographic study of genetic transformation.
Proc Natl Acad Sci U S A. 1968 Aug;60(4):1216-22 PMID: 4386320
Choline-containing teichoic acid as a structural component of pneumococcal cell wall and its role in sensitivity to lysis by an autolytic enzyme.
J Biol Chem. 1970 Jan 25;245(2):287-98 PMID: 4391619
Genetic transformation. I. Cellular incorporation of DNA accompanying transformation in Pneumococcus.
Biochim Biophys Acta. 1957 Oct;26(1):68-82 PMID: 13479462
Autolytic enzyme system of Streptococcus faecalis. 3. Localization of the autolysin at the sites of cell wall synthesis.
J Bacteriol. 1967 Nov;94(5):1525-30 PMID: 4964481
Autolytic activity associated with competent group H streptococci.
J Bacteriol. 1971 Apr;106(1):257-68 PMID: 4928012
Choline in the cell wall of a bacterium: novel type of polymer-linked choline in Pneumococcus.
Science. 1967 Aug 11;157(3789):694-7 PMID: 4381896
Radioautographic evidence for equatorial wall growth in a gram-positive bacterium. Segregation of choline-3H-labeled teichoic acid.
J Cell Biol. 1970 Dec;47(3):786-90 PMID: 4395735
On the nature of the pneumococcal activator substance.
Proc Natl Acad Sci U S A. 1966 Jan;55(1):58-66 PMID: 4380138

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.8.1848

Published

1971-08-00

Pages

1848-52

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389306

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.8.1848 PMC Full Text View journal details

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DNA uptake during genetic transformation and the growing zone of the cell envelope.

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