The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 4 ·1969-12-00 ·Pages 1404-10

Yoch DC, Benemann JR, Valentine RC, Arnon DI

Abstract

A new type of ferredoxin was isolated from Azotobacter vinelandii cells. The protein was able to replace the native chloroplast ferredoxin in the photoreduction of nicotinamide adenine dinucleotide phosphate (NADP) and functioned as a reductant for the Azotobacter nitrogenase.

MeSH Terms

Amino Acids/analysis Azotobacter/metabolism Chromatography, DEAE-Cellulose Electron Transport Ferredoxins/analysis,isolation & purification,metabolism NADP/metabolism Nitrogen Fixation Oxidation-Reduction Oxidoreductases

Chemicals

Amino Acids Ferredoxins NADP Oxidoreductases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Yoch D C

Benemann J R

Valentine R C

Arnon D I

References (17)

17 references, click to expand

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Purification of a non-heme iron protein and other electron transport components from Azotobacter extracts.
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A free radical flavoprotein from Azotobacter. Isolation, crystallization, and properties.
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Spinach ferredoxin. I. Amino acid composition and terminal sequences.
J Biol Chem. 1968 Apr 25;243(8):1725-31 PMID: 5646474
Non heme (iron-sulfur) proteins of Azotobacter vinelandii.
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Enhancement effects and the identity of the two photochemical reactions of photosynthesis.
Proc Natl Acad Sci U S A. 1968 Nov;61(3):989-96 PMID: 4386829
High recovery of tryptophan from acid hydrolysates of proteins.
Biochem Biophys Res Commun. 1969 Apr 29;35(2):175-81 PMID: 5772577
Role of ferredoxin in photosynthesis.
Naturwissenschaften. 1969 Jun;56(6):295-305 PMID: 4391254
The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1079-86 PMID: 5264138
Direct demonstration of ammonia as an intermediate in nitrogen fixation by Azotobacter.
J Biol Chem. 1953 Sep;204(1):445-51 PMID: 13084615
Formyltetrahydrofolate synthetase. I. Isolation and crystallization of the enzyme.
J Biol Chem. 1962 Sep;237:2898-902 PMID: 14489619
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Nature. 1962 Aug 11;195:537-43 PMID: 14039612
STUDIES ON THE CHEMICAL NATURE OF CLOSTRIDIAL FERREDOXIN.
J Biol Chem. 1963 Dec;238:3899-913 PMID: 14086723
FERREDOXIN REQUIREMENT FOR NITROGEN FIXATION BY EXTRACTS OF CLOSTRIDIUM PASTEURIANUM.
Biochim Biophys Acta. 1964 Mar 9;81:473-8 PMID: 14170319
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.
Ann N Y Acad Sci. 1964 Dec 28;121:404-27 PMID: 14240539
BACTERIAL FERREDOXIN.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.4.1404

Published

1969-12-00

Pages

1404-10

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223299

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.4.1404 PMC Full Text View journal details

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The electron transport system in nitrogen fixation by Azotobacter. II. Isolation and function of a new type of ferredoxin.

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