The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 64 ·No. 3 ·1969-11-00 ·Pages 1079-86

Benemann JR, Yoch DC, Valentine RC, Arnon DI

Abstract

A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.

MeSH Terms

Azotobacter/metabolism Chloroplasts/metabolism,radiation effects Chromatography, DEAE-Cellulose Electron Transport Flavins/isolation & purification,metabolism Free Radicals/metabolism Light Nitrogen Fixation Oxidoreductases/metabolism Radiation Effects Sulfites/metabolism

Chemicals

Flavins Free Radicals Sulfites Oxidoreductases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Benemann J R

Yoch D C

Valentine R C

Arnon D I

References (16)

16 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.64.3.1079

Published

1969-11-00

Pages

1079-86

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223346

Subset

External Links

PubMed source DOI: 10.1073/pnas.64.3.1079 PMC Full Text View journal details

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The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

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