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PMID: 4383672 Published · ppublish English Journal Article

Aromatic amino acid biosynthesis: gene-enzyme relationships in Bacillus subtilis.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1706-14

Nasser D, Nester EW

Abstract

Single step mutants of Bacillus subtilis which required either one or all of the aromatic amino acids for growth were isolated. The relevant gene defect was determined for each mutant by enzyme assays in vitro. A mutant deficient in each enzyme step of aromatic amino acid biosynthesis was found with the exceptions of the shikimate kinase and the phenylalanine and tyrosine transaminases. Representative mutants carrying the defective genes were mapped by deoxyribonucleic acid mediated transformation by reference to the aromatic amino acid gene (aro) cluster and, alternately, to any of the other unlinked aro genes. The genes coding for dehydroquinate synthetase, 3-enol pyruvylshikimate 5-phosphate synthetase, one form of chorismate mutase, and prephenate dehydrogenase are linked to the aro cluster. Except for the previously identified linkage between the genes of 3-deoxy-d-arabino heptulosonic acid 7-phosphate synthetase and one species of chorismate mutase, the other genes involved in this pathway are neither linked to the aro cluster nor to each other.

MeSH Terms
Amino Acids/biosynthesis Bacillus subtilis/enzymology,isolation & purification Chromosome Mapping DNA, Bacterial Genes Genes, Regulator Genetic Code Genetics, Microbial Genotype Ligases/metabolism Molecular Biology Mutation Oxidoreductases/metabolism Phenotype Phenylalanine/biosynthesis,metabolism Phosphotransferases/metabolism Shikimic Acid/metabolism Transaminases/metabolism Transformation, Genetic Tryptophan/biosynthesis,metabolism Tyrosine/biosynthesis,metabolism Tyrosine Transaminase/metabolism
Chemicals
Amino Acids DNA, Bacterial Shikimic Acid Tyrosine Phenylalanine Tryptophan Oxidoreductases Transaminases Tyrosine Transaminase Phosphotransferases Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nasser D
Nester E W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.94.5.1706-1714.1967
Published
1967-11-00
Pages
1706-14
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276881
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.94.5.1706-1714.1967 PMC Full Text View journal details
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