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PMID: 4375964 Published · ppublish English Journal Article

Properties of a prolactin receptor from the rabbit mammary gland.

The Biochemical journal ·Vol. 140 ·No. 2 ·1974-05-00 ·Pages 301-11

Shiu RP, Friesen HG

Abstract

Receptors for human, simian, ovine, bovine and murine prolactin, human growth hormone and human placental lactogen have been identified in plasma-membrane-containing subcellular particles isolated from rabbit mammary glands. The association and dissociation of (125)I-labelled prolactin are time- and temperature-dependent processes, both being maximal at 37 degrees C. (125)I-labelled prolactin prepared by the enzymic iodination procedure with lactoperoxidase binds better to receptors than does the preparation obtained by using chloramine-t as the oxidizing agent. The binding of (125)I-labelled prolactin to receptors is strongly influenced by pH and ionic composition but not by many low-molecular-weight compounds tested, e.g. steroids, nucleotides and several drugs. Receptor activity is sensitive to trypsin and phospholipase C digestion, suggesting that protein and phospholipid moieties are essential for the binding of (125)I-labelled prolactin. The binding of (125)I-labelled prolactin to receptors is a saturable and reversible process. Scatchard and Lineweaver-Burk analyses suggest that (125)I-labelled prolactin has a high affinity for its receptor. Binding of (125)I-labelled prolactin to receptors does not result in the destruction of the hormone. Considerable prolactin-binding activity is also observed in subcellular fractions isolated from the adrenal gland, liver, ovary and kidney of the pregnant rabbit, a finding that is consistent with other reported actions of prolactin in these organs.

MeSH Terms
Adrenal Glands/analysis Animals Cattle Female Growth Hormone/metabolism Haplorhini Humans Hydrogen-Ion Concentration Iodine Radioisotopes Kidney/analysis Kinetics Liver/analysis Mammary Glands, Animal/metabolism Mice Ovary/analysis Phospholipases Pregnancy Prolactin/metabolism Protein Binding Rabbits Receptors, Cell Surface Sheep Subcellular Fractions/analysis Temperature Time Factors Trypsin
Chemicals
Iodine Radioisotopes Receptors, Cell Surface Prolactin Growth Hormone Phospholipases Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shiu R P
Friesen H G
References (34)
34 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1400301
Published
1974-05-00
Pages
301-11
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168001
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1400301 PMC Full Text View journal details
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