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PMID: 4315615 Published · ppublish English Journal Article

ACTH receptors in the adrenal: specific binding of ACTH-125I and its relation to adenyl cyclase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 3 ·1970-03-00 ·Pages 745-52

Lefkowitz RJ, Roth J, Pricer W, Pastan I

Abstract

Pure monoiodo ACTH-(125)I was prepared that was biologically active and free of unlabeled ACTH. Extracts of adrenal cortex that contained ACTH-sensitive adenyl cyclase, bound ACTH-(125)I; extracts that lacked the ACTH-sensitive cyclase did not bind ACTH-(125)I. Unlabeled ACTH inhibited the binding of ACTH-(125)I. Five ACTH derivatives which varied widely in biological activity were tested. All inhibited the binding of ACTH-(125)I in direct proportion to their biological activity. Albumin, insulin, and four unrelated iodinated hormones were inert. The addition of excess hormone or acetic acid produced rapid dissociation of bound ACTH-(125)I. This study demonstrates directly the binding of ACTH to its biologically significant site.

MeSH Terms
Adenylyl Cyclases Adrenal Gland Neoplasms/metabolism Adrenal Glands Adrenocorticotropic Hormone Animals Binding Sites Biological Assay Chemoreceptor Cells Iodine Radioisotopes Mice Neoplasms, Experimental/metabolism
Chemicals
Iodine Radioisotopes Adrenocorticotropic Hormone Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lefkowitz R J
Roth J
Pricer W
Pastan I
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.65.3.745
Published
1970-03-00
Pages
745-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282969
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.65.3.745 PMC Full Text View journal details
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