Requirement for cell division for initiation of transcription of Rous sarcoma virus RNA.

Journal of virology ·Vol. 14 ·No. 3 ·1974-09-00 ·Pages 531-46

Humphries EH, Temin HM

Abstract

Stationary chicken embryo fibroblasts exposed to Rous sarcoma virus (RSV) did not synthesize virus RNA until after division of the infected cells. Initiation of RNA transcription after cell division resulted in transcription of the entire viral genome and was followed shortly thereafter by appearance of progeny virus. Addition of colchicine to RSV-infected stationary chicken embryo fibroblasts resulted in inhibition of cell division and a 24-h delay in the appearance of virus RNA. RSV-infected chicken embryo fibroblasts made stationary 10 to 15 cell generations after infection continued to produce an amount of progeny virus similar to that produced in dividing RSV-infected cells. Teh virus produced from cells made stationary after virus production had begun contained RNA synthesized in stationary cells. Therefore, division of the RSV-infected chicken embryo fibroblast is required for the initiation of transcription of virus RNA, but it is not required for the maintenance of transcription of virus RNA.

MeSH Terms

Animals Avian Sarcoma Viruses/growth & development Blood Cell Division Cells, Cultured Chick Embryo Colchicine/pharmacology Fibroblasts In Vitro Techniques Iodine Radioisotopes Nucleic Acid Hybridization Phosphorus Radioisotopes RNA, Viral/biosynthesis Thymidine Time Factors Transcription, Genetic Tritium Uridine Virus Replication/drug effects

Chemicals

Iodine Radioisotopes Phosphorus Radioisotopes RNA, Viral Tritium Colchicine Thymidine Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Humphries E H

Temin H M

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26 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.14.3.531-546.1974

Published

1974-09-00

Pages

531-46

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355547

Subset

External Links

PubMed source DOI: 10.1128/JVI.14.3.531-546.1974 PMC Full Text View journal details

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Requirement for cell division for initiation of transcription of Rous sarcoma virus RNA.

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