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PMID: 4362642 Published · ppublish English Journal Article

Identification of a high affinity nuclear acceptor site for estrogen receptor of calf uterus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 979-83

Puca GA, Sica V, Nola E

Abstract

By means of affinity chromatography, specific nuclear acceptor sites for estradiol receptors are identified in a fraction that can be solubilized from purified nuclei with 2 M NaCl. Interaction between these acceptor sites and crude or partially purified estradiol receptor shows a high association constant (over 10(9) M). Receptor-acceptor interaction is dependent on physiological concentrations of 17beta-estradiol; it is disrupted by high ionic strength. The nuclear acceptor sites appear to be protein in nature and exist in 5- to 10-fold excess over the estrogen binding sites present in the cytosol. Single- or double-stranded DNA does not bind estrogen-receptor complexes. Acceptor sites appear to be associated with basic nuclear proteins as judged by hydroxyapatite chromatography. The nuclear acceptor sites probably represent less than 0.1% of the purified basic proteins from the nucleus.

MeSH Terms
Animals Binding Sites Cattle Cell Nucleus/metabolism Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange DNA Deoxyribonucleases/metabolism Estradiol/metabolism Female Hydroxyapatites Nucleoproteins/isolation & purification,metabolism Polysaccharides Pronase/metabolism Receptors, Cell Surface Ribonucleases/metabolism Tritium Trypsin/metabolism Uterus/cytology,metabolism
Chemicals
Hydroxyapatites Nucleoproteins Polysaccharides Receptors, Cell Surface Tritium Estradiol DNA Deoxyribonucleases Ribonucleases Trypsin Pronase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Puca G A
Sica V
Nola E
References (38)
38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.3.979
Published
1974-03-00
Pages
979-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388141
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.3.979 PMC Full Text View journal details
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