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PMID: 4315614 Published · ppublish English Journal Article

The relationship between glucocorticoid binding and tyrosine aminotransferase induction in hepatoma tissue culture cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 65 ·No. 3 ·1970-03-00 ·Pages 709-15

Baxter JD, Tomkins GM

Abstract

Glucocorticoid receptors with many properties suggesting their involvement in the hormonal induction of tyrosine aminotransferase have been detected in rat hepatoma tissue culture cells. These "specific receptors" approach saturation as the steroid concentrations required for maximal induction are reached. Inducer analogs influence cortisol binding to the specific receptors in direct relation to their influence on cortisol induction. The inducer association with and dissociation from specific receptors are rapid enough to account for the kinetics of induction and deinduction. Chromatographic studies suggest the specific receptors bind unaltered dexamethasone and cortisol. "Nonspecific" association of steroids with the cells also occurs and can be distinguished from specific binding.

MeSH Terms
Binding Sites Carbon Isotopes Carcinoma, Hepatocellular/enzymology Culture Techniques Dexamethasone/pharmacology Enzyme Induction/drug effects Glucocorticoids/pharmacology Hydrocortisone/pharmacology Liver Neoplasms Neoplasms, Experimental/enzymology Transaminases Tritium
Chemicals
Carbon Isotopes Glucocorticoids Tritium Dexamethasone Transaminases Hydrocortisone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baxter J D
Tomkins G M
References (21)
21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.65.3.709
Published
1970-03-00
Pages
709-15
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282964
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.65.3.709 PMC Full Text View journal details
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