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PMID: 4362513 Published · ppublish English Journal Article

Fate of mRNA of L-cells infected with mengovirus.

Journal of virology ·Vol. 13 ·No. 4 ·1974-04-00 ·Pages 858-69

Colby DS, Finnerty V, Lucas-Lenard J

Abstract

Mengovirus infection of L-cells results in an inhibition of host protein synthesis which is detectable in vivo by a decreased rate of incorporation of radioactive amino acids into acid-insoluble material and by a concomitant reduction in polysome content. The inhibition of host protein synthesis occurs early in the infection cycle, at a time when there is little synthesis of viral proteins. In this paper the stability of polyadenylic acid [poly(A)]-containing mRNA of uninfected L-cells and cells infected with mengovirus is compared. Our results suggest that there is no increase in the rate of degradation of cellular mRNA upon virus infection. The continued integrity of host mRNA throughout infection was verified by acrylamide gel electrophoresis.

MeSH Terms
Amino Acids/metabolism Animals Centrifugation, Density Gradient Deoxyadenosines/pharmacology Electrophoresis, Polyacrylamide Gel L Cells/drug effects Mengovirus/drug effects,growth & development Mice Polynucleotides/analysis Protein Biosynthesis RNA, Messenger/analysis RNA, Viral/analysis Tritium Uridine
Chemicals
Amino Acids Deoxyadenosines Polynucleotides RNA, Messenger RNA, Viral Tritium Uridine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Colby D S
Finnerty V
Lucas-Lenard J
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.4.858-869.1974
Published
1974-04-00
Pages
858-69
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355384
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.4.858-869.1974 PMC Full Text View journal details
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