Cell-surface mucosubstances from trypsin diaggregation of normal and virus-transformed lines of baby-hamster kidney cells.

The Biochemical journal ·Vol. 134 ·No. 4 ·1973-08-00 ·Pages 1123-6

Minnikin SM, Allen A

Abstract

Cell disaggregation by trypsin solubilizes significantly less mucosubstance from the surface of polyoma-virus-transformed baby-hamster kidney cells than from the same non-transformed cell line. The mucosubstance, which consists of both acid mucopolysaccharides and mucoproteins, also differs qualitatively in the two cell lines.

MeSH Terms

Animals Cell Line Cell Membrane/metabolism Cell Transformation, Neoplastic Cricetinae Culture Media Culture Techniques Galactosamine/isolation & purification Glucosamine/isolation & purification Glycosaminoglycans/metabolism Hexosamines/isolation & purification Hydrolysis Kidney Mucoproteins/metabolism Neuraminic Acids/isolation & purification Polyomavirus Sulfur Radioisotopes Tritium Trypsin/pharmacology

Chemicals

Culture Media Glycosaminoglycans Hexosamines Mucoproteins Neuraminic Acids Sulfur Radioisotopes Tritium Galactosamine Trypsin Glucosamine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Minnikin S M

Allen A

References (25)

25 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1341123

Published

1973-08-00

Pages

1123-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177922

Subset

External Links

PubMed source DOI: 10.1042/bj1341123 PMC Full Text View journal details

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Cell-surface mucosubstances from trypsin diaggregation of normal and virus-transformed lines of baby-hamster kidney cells.

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