Separation of DNA sequences complementary to the RNA of avian myeloblastosis virus from chicken DNA by alkaline cesium chloride density sedimentation.

Journal of virology ·Vol. 12 ·No. 3 ·1973-09-00 ·Pages 534-7

Shoyab M, Baluda MA

Abstract

Density gradient sedimentation in alkaline cesium chloride of DNA from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13S revealed that the DNA sequences complementary to 70S avian myeloblastosis virus RNA sedimented in the high guanine plus cytosine region ahead of the main peak of cellular DNA. When the DNA was fragmented into pieces of 6.6S there was a broader distribution of the DNA sequences complementary to the viral RNA. This technique could be employed as a step towards the isolation of DNA copies of the entire viral RNA genome from the mass of host cellular DNA.

MeSH Terms

Animals Avian Leukosis/metabolism Avian Leukosis Virus/analysis Base Sequence Bone Marrow/analysis Bone Marrow Cells Centrifugation, Density Gradient Cesium Chick Embryo Chlorides Cytosine/analysis DNA/analysis,isolation & purification DNA, Neoplasm/analysis,isolation & purification DNA, Viral/isolation & purification Guanine/analysis Molecular Weight Nucleic Acid Hybridization RNA, Viral/analysis,isolation & purification Transcription, Genetic Tritium

Chemicals

Chlorides DNA, Neoplasm DNA, Viral RNA, Viral Tritium Cesium Guanine Cytosine DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shoyab M

Baluda M A

References (13)

13 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.12.3.534-537.1973

Published

1973-09-00

Pages

534-7

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356659

Subset

External Links

PubMed source DOI: 10.1128/JVI.12.3.534-537.1973 PMC Full Text View journal details

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Separation of DNA sequences complementary to the RNA of avian myeloblastosis virus from chicken DNA by alkaline cesium chloride density sedimentation.

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