DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 2 ·1970-06-00 ·Pages 329-36

Baluda MA, Nayak DP

Abstract

Nucleic acid hybridization studies were made between 71S-AMV-RNA and DNA from leukemic myeloblasts and from normal chicken cells. There was homology between the viral RNA and chicken cell DNA and to a greater extent between viral RNA and leukemic cell DNA. Leukemic cell DNA hybridized approximately twice as much viral RNA as did normal chicken DNA. Thermal melting studies showed that the viral RNA bound to normal and leukemic cell DNA consists of long polynucleotides (T(m) = 87 degrees and 92 degrees C, respectively, in 2x saline citrate). This suggests that the leukemic cells contain a DNA template of the viral RNA.

MeSH Terms

Animals Avian Leukosis Virus Chick Embryo Chromatography Culture Techniques DNA DNA, Viral Hot Temperature Hybridization, Genetic RNA, Viral Templates, Genetic Tritium

Chemicals

DNA, Viral RNA, Viral Tritium DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baluda M A

Nayak D P

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.2.329

Published

1970-06-00

Pages

329-36

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283048

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.2.329 PMC Full Text View journal details

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DNA complementary to viral RNA in leukemic cells induced by avian myeloblastosis virus.

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