Antigenic properties of endogenous type-C viruses from spontaneously transformed clones of BALB-3T3.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1598-602

Aoki T, Todaro GJ

Abstract

During long-term tissue culture of spontaneously transformed clones from BALB/c 3T3 mouse-embryo cells, some clones spontaneously begin to produce high titers of endogenous murine type-C viruses. The antigenic properties of these viruses have been analyzed by indirect immunoelectronmicroscopy and can be classified into two distinguishable populations: (a) BALB/c murine myeloma-associated extracellular viruses that carry a specific envelope antigen, xVEA, different from the typical murine leukemia viral envelope antigens; and (b) previously uncharacterized type-C viruses that have neither xVEA nor the murine leukemia viral envelope antigens. The former produces PC1 antigen and the latter might induce a new cell-surface antigen. Neither of these two populations of BALB/3T3 endogenous type-C viruses was able to infect BALB/c cells but both could infect NIH Swiss cells. A single BALB/3T3 clone, then, can release infectious endogenous type-C viruses with at least two different antigenic properties. We conclude that BALB/c somatic cells contain preexisting genetic information for production of at least closely related but, nevertheless, distinct type-C viruses.

MeSH Terms

Animals Antibodies, Viral Antigens, Viral Cell Membrane/immunology Cell Transformation, Neoplastic Clone Cells Embryo, Mammalian Ferritins Fibroblasts Gammaretrovirus/immunology Genes Immune Sera Mice Mice, Inbred Strains Microscopy, Electron Multiple Myeloma/microbiology Neoplasms, Experimental/microbiology Oncogenic Viruses/immunology RNA Viruses/immunology Retroviridae/immunology

Chemicals

Antibodies, Viral Antigens, Viral Immune Sera Ferritins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Aoki T

Todaro G J

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.5.1598

Published

1973-05-00

Pages

1598-602

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433550

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.5.1598 PMC Full Text View journal details

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Antigenic properties of endogenous type-C viruses from spontaneously transformed clones of BALB-3T3.

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