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PMID: 4347595 Published · ppublish English Journal Article

Radioimmunoassay of mammalian type C viral proteins. 3. Detection of viral antigen in normal murine cells and tissues.

The Journal of experimental medicine ·Vol. 137 ·No. 3 ·1973-03-01 ·Pages 622-35

Parks WP, Livingston DM, Todaro GJ, Benveniste RE, Scolnick EM

Abstract

A radioimmunoassay specific for a murine leukemia virus structural protein, the gs antigen, detects an antigenic reactivity in normal murine cells in culture and natural tissues. The assay was shown to measure an antigen that is highly related to the virion protein as shown by absorption tests, immunoadsorbent chromatography, and by analysis of linearized dose-response curves. These findings combined with the finding of viral-specific RNA indicate that portions of the viral genome are being expressed with a much greater frequency than previously appreciated.

MeSH Terms
Animals Antigens, Viral/analysis Cell Line Cells, Cultured/analysis Chromatography, Affinity Female Humans Iodine Isotopes Kidney/immunology Leukemia Virus, Murine/immunology Liver/immunology Lymphoid Tissue/immunology Male Mice Mice, Inbred Strains Nucleic Acid Hybridization RNA, Viral/analysis Radioimmunoassay Rats Rauscher Virus/immunology Retroviridae/immunology Thymidine Tritium Viral Proteins/analysis
Chemicals
Antigens, Viral Iodine Isotopes RNA, Viral Viral Proteins Tritium Thymidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Parks W P
Livingston D M
Todaro G J
Benveniste R E
Scolnick E M
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35 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.137.3.622
Published
1973-03-01
Pages
622-35
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139374
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.137.3.622 PMC Full Text View journal details
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