Inhibition of cleavage of large poliovirus-specific precursor proteins in infected HeLa cells by inhibitors of proteolytic enzymes.

Journal of virology ·Vol. 10 ·No. 4 ·1972-10-00 ·Pages 880-4

Summers DF, Shaw EN, Stewart ML, Maizel JV

Abstract

Inhibitors of chymotrypsin interfere with the post-translational cleavage of large poliovirus-specific polypeptides in the molecular weight range of 100,000 to 250,000 in infected HeLa cells.

MeSH Terms

Autoradiography Carbon Isotopes Chymotrypsin/antagonists & inhibitors Electrophoresis, Polyacrylamide Gel Genetics, Microbial HeLa Cells/metabolism Humans Ketones/pharmacology Methionine/metabolism Molecular Weight Peptide Biosynthesis Peptides/analysis Poliovirus/analysis,growth & development,metabolism Protein Biosynthesis Stereoisomerism Sulfur Isotopes Trypsin Inhibitors/pharmacology Uridine/metabolism Viral Proteins/analysis,biosynthesis

Chemicals

Carbon Isotopes Ketones Peptides Sulfur Isotopes Trypsin Inhibitors Viral Proteins Methionine Chymotrypsin Uridine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Summers D F

Shaw E N

Stewart M L

Maizel J V

References (18)

18 references, click to expand

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Effect of temperature on the cleavage of polypeptides during growth of LSc poliovirus.
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Comparison of polysomal structures of uninfected and poliovirus infected HeLa cells.
Virology. 1971 May;44(2):239-48 PMID: 4327714
Active-site-directed phenacyl halides inhibitory to trypsin.
Arch Biochem Biophys. 1971 Jan;142(1):340-50 PMID: 5545488
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Proc Natl Acad Sci U S A. 1971 Nov;68(11):2852-6 PMID: 4330946
Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.
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Molecular weight of poliovirus ribonucleic acid.
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Selective inhibition of the synthesis of Sindbis virion proteins by an inhibitor of chymotrypsin.
J Virol. 1972 Jan;9(1):187-8 PMID: 5061988
Evidence for large precursor proteins in poliovirus synthesis.
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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.10.4.880-884.1972

Published

1972-10-00

Pages

880-4

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC356549

Subset

External Links

PubMed source DOI: 10.1128/JVI.10.4.880-884.1972 PMC Full Text View journal details

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Inhibition of cleavage of large poliovirus-specific precursor proteins in infected HeLa cells by inhibitors of proteolytic enzymes.

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